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Gene and miRNA expression profiling in pediatric and adolescent and young adult rhabdomyosarcomas

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Homo sapiens

PROVIDER: GSE135519 | GEO | 2019/11/05

REPOSITORIES: GEO

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miRNA expression profile in pediatric and adolescent and young adult rhabdomyosarcomas

Project description:Adolescents and young adults (AYA) with rhabdomyosarcoma (RMS) form a subgroup of patients whose optimal clinical management and best possible access to care remain a challenge and survival lacks behind that of children while diagnosed with histologically similar tumors. Understanding the tumor biology that differentiate children from AYA-RMS could provide critical information and drive new initiatives to improve their final outcome. MiRNA profile was evaluated in a RMS series of 49 tumor and 13 non-neoplastic tissues. MiRNAs analysis identified miR-223 over-expression and mir-431 down-regulation in AYA, validated by Real-Time PCR and miRNA ISH.

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Gene expression profiling in pediatric and adolescent and young adult rhabdomyosarcomas

Project description:Adolescents and young adults (AYA) with rhabdomyosarcoma (RMS) form a subgroup of patients whose optimal clinical management and best possible access to care remain a challenge and survival lacks behind that of children while diagnosed with histologically similar tumors. Understanding the tumor biology that differentiate children from AYA-RMS could provide critical information and drive new initiatives to improve their final outcome. Gene expression profiling was evaluated in a RMS series of 48 tumor and 13 non-neoplastic tissues. GEP analysis detected 793 age-correlated genes in tumors. NOTCH2, FGFR1 were significantly down-modulated in AYA-RMS. GEP showed an increase in CD4 memory resting cells and a decrease in γδ T-cells in AYA-RMS. IHC analysis demonstrated an increase of infiltrated CD4, CD8 and neutrophils in AYA-RMS tumors.

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