Transcriptomics

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Probe-Seq enables transcriptional profiling of specific cell types from heterogeneous tissue by RNA-based isolation


ABSTRACT: Probe-Seq is a method that allows transcriptional profiling of specific cell types from heterogeneous tissue by labeling RNA. Briefly, we developed Probe-Seq, which allows deep transcriptional profiling of specific cell types isolated based on RNA abundance of defined markers. We applied Probe-Seq to purify and profile specific cell types from mouse, human, and chick retinas as well as the Drosophila gut. We also showed that Probe-Seq is compatible with frozen nuclei and that multiplexed Probe-Seq enables iterative isolation of multiple cell types from the same tissue sample. Provided that unique markers are available, this simple, novel method could potentially enable bulk RNA sequencing of any cell type from any organism.

ORGANISM(S): Mus musculus Gallus gallus Drosophila melanogaster

PROVIDER: GSE135572 | GEO | 2019/12/09

REPOSITORIES: GEO

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