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Transcriptome profiling of embryonic development rate in rainbow trout advance backcross introgression lines

ABSTRACT: Embryonic development rate is a key trait significantly associated and genetically linked with both growth rate and sexual maturity in rainbow trout. To identify candidate genes underlying embryonic development rate, whole genome expression microarray analyses were conducted using embryos from a fourth generation backcross family; each backcross generation involved the introgression of the fast-developing alleles for the major development rate QTL (from the Clearwater clonal line [CW]) into a slow-developing clonal line (Oregon State University [OSU]). Embryos were collected at 15, 19, and 28 days post-fertilization. Microsatellite markers (One112ADFG, OMM1009 and OmyFGT12TUF) linked to the major embryonic development rate QTL region were used to determine the QTL genotype. The sex marker OMY-Y1 was used to determine the genotypic sex of each embryo. cDNA from 48 individual embryos were used for microarray expression analysis. An ANOVA modeling approach was used to detect differential gene expression between embryos possessing fast-developing genotypes and slow-developing genotypes. A total of 182 features have been identified with significant differences between embryonic development rate genotypes. Quantitative PCR was conducted on ten representative genes using the rainbow trout homologous sequences and microarray results were validated.

ORGANISM(S): Salmo salar Oncorhynchus tshawytscha Osmerus mordax Coregonus clupeaformis Oncorhynchus mykiss

PROVIDER: GSE13570 | GEO | 2009/11/12

SECONDARY ACCESSION(S): PRJNA110675

REPOSITORIES: GEO

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Transcriptome profiling of embryonic development rate in rainbow trout advance backcross introgression lines

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