Dataset Information

Proteomic identification of rainbow trout sperm proteins

ABSTRACT: Proteomics represents a powerful tool for the analysis of fish spermatozoa, since these cells are transcriptionally inactive. The aim of the present study was to generate an inventory of the most prominent rainbow trout sperm proteins with the use of one-dimensional electrophoresis prefractionation combined with performance liquid chromatography electrospray ionization tandem mass spectrometry. This study provides the first in-depth analysis of the rainbow trout sperm proteome, with a total of 204 identified proteins. We found that rainbow trout spermatozoa are equipped with functionally diverse proteins related to energetic metabolism, signal transduction, protein turnover, transport, cytoskeleton, oxidative injures and stress and reproduction. The availability of a catalogue of rainbow trout sperm proteins provides a crucial tool for the understanding of fundamental molecular processes in fish spermatozoa for ongoing research in the development of novel markers of sperm quality and for the optimization of short- and long-term sperm preservation procedures.

INSTRUMENT(S): LTQ

ORGANISM(S): Oncorhynchus Mykiss (rainbow Trout) (salmo Gairdneri)

TISSUE(S): Spermatozoon

SUBMITTER: Joanna Nynca

LAB HEAD: Joanna Nynca

PROVIDER: PXD000355 | Pride | 2013-11-04

REPOSITORIES: Pride

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			Action	DRS
	PRIDE_Exp_Complete_Ac_31933.pride.mgf.gz	Mgf
	PRIDE_Exp_Complete_Ac_31933.pride.mztab.gz	Mztab
	PRIDE_Exp_Complete_Ac_31933.xml.gz	Xml
	PRIDE_Exp_Complete_Ac_31934.pride.mgf.gz	Mgf
	PRIDE_Exp_Complete_Ac_31934.pride.mztab.gz	Mztab

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Proteomic identification of rainbow trout sperm proteins.

Nynca Joanna J Arnold Georg J GJ Fröhlich Thomas T Otte Kathrin K Ciereszko Andrzej A

Proteomics 20140512 12

Proteomics represents a powerful tool for the analysis of fish spermatozoa, since these cells are transcriptionally inactive. The aim of the present study was to generate an inventory of the most prominent rainbow trout sperm proteins by SDS-PAGE prefractionation combined with nano-LC-MS/MS based identification. This study provides the first in-depth analysis of the rainbow trout sperm proteome, with a total of 206 identified proteins. We found that rainbow trout spermatozoa are equipped with fu ...[more]

PMID: 24687995

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