Transcriptomic signatures of spleen and liver CD4+ T cells from Leishmania donovani-infected C57BL/6J mice
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ABSTRACT: This study was designed to investigate tissue-specific transcriptional changes in spleen and liver CD4+ T cells from C57BL/6J mice infected with L. donovani.
Project description:Monastrol treatment of Leishmania donovani infected macrophages Macrophages were infected with Leishmania donovani and treated with monastrol to look for signalling molecules
Project description:In the context of studying visceral leishmaniasis, neutrophils infected with Leishmania donovani have been compared to uninfected neutrophils. Compared time points are 0, 6 and 24 hours post infection. Neutrophils of three human donors have been used. Overall 6 samples for infected neutrophils at time point 6 hours and 6 samples for infected neutrophils at time point 24 hours exist, including three biological samples and two technical samples. Uninfected neutrophils represent 3 samples at time point 0 hours, 3 samples at time point 6 hours and 3 samples at time point 24 hours. Transcriptome of Leishmania donovani culture has been assessed in two replicates.
Project description:Murine bone marrow derived macrophages were infected with Leishmania major or Leishmania donovania promastigotes, allowed to phagocytose latex beads or not treated. Gene expression profiles were compared to identify i) the effect of Leishmania infection; ii) the differences in effects between L. major and L. donovani; and iii) the effect of pahgocytosis of latex beads.
Project description:FIve 6/8 week old female BALB/c mice were infected with the LV9 L. donovani strain,five mice were infected with the L. infantum NCL strain, five were uninfected, and spleen and liver were taken from mice at day 36 post infection. These tissues were then used to prepare for RNAseq