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Dissection of FUS domains involved in regulation of SnRNP70 gene expression

ABSTRACT: To identify FUS regulated genes and its dependency of snRNP70, the gene expression profiles in neurons expressing the Gly-rich domain deleted FUS mutant coupled with Snrnp70 gene knock down were analyzed.

ORGANISM(S): Mus musculus

PROVIDER: GSE136663 | GEO | 2020/09/17

REPOSITORIES: GEO

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Dissection of FUS domains involved in regulation of SnRNP70 gene expression

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Project description:The aim of our study is to identify the role of FUS in shaping the transcriptome. RNA-seq of two FUS KO clones was performed and compared to wt; for each, four replicates were sequenced. RNA molecules associated with the FUS protein were determined by means of a RNA immuno-precipitation, followed by high-throughput sequencing. Total RNA was used as a control. SH-SY5Y cells were used for both experiments. RNA-seq: 4 wt samples, 4 A4 KO samples, 4 A5 KO samples. RIP-seq: 1 input control sample, 3 anti-FUS IP replicates.

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