Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Impact of the bone microenvironment on phenotypic plasticity of ER+ breast cancer cells [MCF7]

ABSTRACT: Translating Ribosome Affinity Purification (TRAP) sequencing revealed that human osteogenic cells (FOB1.19) strongly alter the transcriptional profile of ER+ breast cancer cells (MCF7) in 3D coculture system.

ORGANISM(S): Homo sapiens

PROVIDER: GSE137270 | GEO | 2021/04/16

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

L1CAM dependent transcriptional programs in brain metastatic cells

Project description:L1CAM dependent gene expression programs in vitro and ex vivo were analyzed by translating ribosome affinity purification and sequencing (TRAP-Seq).

2018-07-23 | GSE82281 | GEO

Impact of the bone microenvironment on phenotypic plasticity of ER+ breast cancer cells[Osteogenic cells]

Project description:RNA sequencing was used to evaluate the effect of cancer cells on osteogenic cells (FOB 1.19) in presence of estrogen or androgen. Here, we unbiasely evaluated the expression level of fibroblast growth factor receptors (FGFs) in osteogenic cells (FOB) alone.

2021-04-16 | GSE137245 | GEO

Central Amygdala TRAP-Seq of Drd2 neurons

Project description:Sequencing actively translating transcripts of Drd2 neurons using translating ribosome affinity purification (TRAP identifies differentially regulated mRNAs following fear learning. Differentially expressed transcripts with potentially targetable gene products include Npy5r, Rxrg, Adora2a, Sst5r, Fgf3, ErbB4, Fkbp14, Dlk1, and Ssh3.

2019-07-24 | GSE114784 | GEO

TRAP-seq of VMH Nr5a1 neurons

Project description:To identify transcripts enriched in ventomedial hypothalamus (VMH) neurons, we used translating affinity ribosome purification combined with RNA-seq (TRAP-seq) from Nr5a1-Cre mice.

2021-05-19 | GSE172206 | GEO

TRAP-seq of VMH Lepr neurons

Project description:To identify transcripts enriched in ventomedial hypothalamus (VMH) Lepr neurons, we used translating affinity ribosome purification combined with RNA-seq (TRAP-seq) from Lepr-Cre mice.

2021-05-19 | GSE172200 | GEO

ER-RIME in wt-MCF7 and MCF7-LTED

Project description:Wild type (wt) MCF7 cells, modelling breast cancer at primary diagnosis, were cultured in phenol red-free RPMI supplemented with 10% FBS and 1nM estradiol (E2). Long-term oestrogen deprived (LTED) cell lines, which model resistance to endocrine therapy, were cultured in phenol red-free RPMI in the absence of exogenous E2 and supplemented with 10% dextran charcoal-stripped bovine serum (DCC). Samples were harvested at baseline and at the point of resistance (LTED). In order to do comparative analysis in the ER-interactome of wt-MCF7 and MCF7-LTED cells, ER-RIME (rapid immunoprecipitation mass spectrometry of endogenous proteins) was conducted in these cells.

2020-07-13 | PXD004648 | Pride

Reprogramming of root cells during nitrogen-fixing symbiosis entails dynamic polysome association of coding and non-coding RNAs

Project description:we compared changes in the transcriptome and the translatome of M. truncatula roots upon rhizobial infection using direct RNA-seq and Translating Ribosome Affinity Purification (TRAP) combined with RNA-seq (TRAP-seq), respectively. TRAP is a ribosome immunopurification approach that has been extensively used in plants and mammals to assess translational changes

2019-11-15 | GSE133510 | GEO

TRAP-seq of VMH Lepr∩Slc17a6 neurons

Project description:To identify transcripts enriched in ventomedial hypothalamus (VMH) neurons that contain both Lepr and Slc17a6, we used translating affinity ribosome purification combined with RNA-seq (TRAP-seq) from Lepr-Cre;Slc17a6-Flpo mice.

2021-05-19 | GSE172202 | GEO

Gene expression profiling of inputs to the mesolimbic dopamine circuit

Project description:PRV-Circuit-TRAP of DAT-cre mice injected with PRV-Introvert-GFP in the nucleus accumbens These studies identify important inputs to the mesolimbic dopamine pathway and further show that PRV circuit-directed translating ribosome affinity purification (PRV-Circuit-TRAP) can be broadly applied to identify molecularly defined neurons comprising complex, multisynaptic circuits.

2017-03-20 | GSE95234 | GEO

Profiling the endothelial translatome in vivo using ‘AngioTag’ zebrafish

Project description:We profiled the gene expression patterns of undisturbed endothelial cells in living animals using a novel ‘AngioTag’ zebrafish transgenic line that permits isolation of actively translating mRNAs from endothelial cells in their native environment. This transgenic line uses the endothelial cell-specific kdrl promoter to drive expression of an epitope tagged Rpl10a 60S ribosomal subunit protein, allowing for Translating Ribosome Affinity Purification (TRAP) of actively translating endothelial cell mRNAs. We also collected the whole embryo translatome using the TRAP protocol and a ubiquitously expressed tagged Rpl10a, and the endothelial transcritome by collecting the GFP+ endothelial cells that had the kdrl promoter driving GFP.

2019-03-29 | GSE121973 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data