Genomics

Dataset Information

0

Widespread targeting of nascent transcripts by RsmA in Pseudomonas aeruginosa


ABSTRACT: In the opportunistic pathogen Pseudomonas aeruginosa RsmA is an RNA-binding protein that plays critical roles in the control of virulence, interbacterial interactions and biofilm formation. Although RsmA is thought to exert its regulatory effects by binding full-length transcripts, the extent to which RsmA binds nascent transcripts has not been addressed. Moreover, which transcripts are direct targets of this key post-transcriptional regulator is largely unknown. Using chromatin immunoprecipitation coupled with high-throughput DNA sequencing, with cells grown in the presence and absence of the RNA polymerase inhibitor rifampicin, we identify hundreds of nascent transcripts that RsmA associates with in P. aeruginosa. We also find that the RNA chaperone Hfq targets a subset of the RsmA-associated nascent transcripts and that the two RNA-binding proteins can exert regulatory effects on common targets. Our findings establish that RsmA associates with many transcripts as they are being synthesized in P. aeruginosa, identify the direct targets of RsmA, and suggest that RsmA and Hfq may act in a combinatorial fashion on certain target transcripts. More broadly, our data suggest that the binding of post-transcriptional regulators to nascent transcripts may be commonplace in bacteria where distinct regulators can function alone or in concert to achieve control over the translation of transcripts as soon as they emerge from RNA polymerase.

ORGANISM(S): Pseudomonas aeruginosa PAO1

PROVIDER: GSE138338 | GEO | 2020/04/20

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2018-05-01 | GSE111037 | GEO
2018-05-01 | GSE94113 | GEO
| PRJNA575528 | ENA
2006-09-15 | GSE4485 | GEO
2019-11-15 | GSE136111 | GEO
2019-11-15 | GSE136110 | GEO
2007-10-27 | E-GEOD-4485 | biostudies-arrayexpress
2022-02-07 | GSE171056 | GEO
2017-07-21 | PXD003360 | Pride
2016-05-25 | GSE74425 | GEO