Other

Dataset Information

0

Enhancers predominantly control transcription initiation [Capture-C]


ABSTRACT: Gene transcription occurs via a cycle of linked events including initiation, promoter proximal pausing and elongation of RNA polymerase II (Pol 2). A key question is how do transcriptional enhancers influence these events to control gene expression? Here we have used a new approach to quantify transcriptional initiation and pausing in-vivo, while simultaneously identifying transcription start sites (TSSs) and pause-sites (TPSs). When analysed in parallel with nascent RNA-seq, these data show that differential gene expression is achieved predominantly via changes in transcription initiation rather than Pol 2 pausing or elongation. Using genetically engineered mouse models deleted for specific enhancers we show that these elements control gene expression via Pol 2 recruitment and/or initiation rather than via promoter proximal pause release. Using genome-wide analysis we show that enhancers, in general, control gene expression at the stage of Pol 2 recruitment and initiation rather than via pausing.

ORGANISM(S): Mus musculus

PROVIDER: GSE138353 | GEO | 2021/02/03

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2021-02-03 | GSE138358 | GEO
2021-02-03 | GSE138357 | GEO
2021-02-03 | GSE138356 | GEO
2021-02-03 | GSE138355 | GEO
2021-02-03 | GSE138354 | GEO
2024-05-31 | PXD037842 | Pride
2015-06-15 | E-GEOD-65687 | biostudies-arrayexpress
2015-06-15 | E-GEOD-65688 | biostudies-arrayexpress
2014-01-14 | E-GEOD-51633 | biostudies-arrayexpress
2010-04-02 | E-GEOD-20530 | biostudies-arrayexpress