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Influence of varying dietary ratios of ω6 to ω3 fatty acids on the hepatic global gene expression, and association with phenotypic traits (growth, somatic indices and tissue lipid composition) in Atlantic salmon (Salmo salar)

ABSTRACT: The present study investigated the influence of plant-based diets (12-week exposure) with varying ω6:ω3 (0.4–2.7) on the hepatic transcriptome of Atlantic salmon, and the relationships with tissue (i.e. liver, muscle) lipid composition, and growth performance. The current microarray study identified several genes involved in immune and inflammatory response (lect2, itgb5, p43), lipid metabolism (helz2), cell growth (htra1), muscle development (mef2d), and translation (eif2a, eif4b1, p43), that were differentially expressed between the two extreme ω6:ω3 dietary treatments (i.e. high ω6 vs. high ω3).

ORGANISM(S): Salmo salar

PROVIDER: GSE139418 | GEO | 2021/06/23

REPOSITORIES: GEO

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The molecular basis of essential fatty acid limitation in Daphnia magna: A transcriptomic approach

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Mouse peritoneal macrophages at 3.5 or 48 hours, 0,0.5 or 5 μg/ml Leukocyte cell-derived chemotaxin 2 (LECT2),gene expression array, two independent biological replicates

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Mouse peritoneal macrophages at 3.5 or 48 hours, 0,0.5 or 5 μg/ml Leukocyte cell-derived chemotaxin 2 (LECT2),gene expression array, two independent biological replicates

Project description:LECT2 is a liver derived cytokine and involved in many pathologic conditions, especially in immune regulation. To better understand cellular and molecular mechanisms of LECT2 in immune response, mouse peritoneal macrophages were isolated from mouse peritonaeum. After treatment with 0, 0.5 or 5 μg/ml of LECT2 for 3.5 or 48 hours, gene expression profile in mouse peritoneal macrophages was measured using gene expression array. Results showed that LECT2 treatment led to the enhancement of cytokines secretion and phagocytosis in peritoneal macrophages. Gene expression in mouse peritoneal macrophages isolated from mouse peritonaeum were measured after exposure to 0,0.5 or 5 μg/ml LECT2 for 3.5 or 48 hours using gene expression array. Two independent experiments were performed using different mice for each experiment for gene expression array analysis.

2011-12-13 | E-GEOD-33721 | biostudies-arrayexpress

Ayu macrophages at 3.5 hours, 0, 0.5 or 5 ug/ml Leukocyte cell-derived chemotaxin 2 (LECT2), gene expression array, three independent biological replicates

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2012-08-21 | E-GEOD-40221 | biostudies-arrayexpress

Influence of varying dietary ratios of ω6 to ω3 fatty acids on the hepatic global gene expression, and association with phenotypic traits (growth, somatic indices and tissue lipid composition) in Atlantic salmon (Salmo salar)

Project description:Influence of varying dietary ratios of ω6 to ω3 fatty acids on the hepatic global gene expression, and association with phenotypic traits (growth, somatic indices and tissue lipid composition) in Atlantic salmon (Salmo salar)

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