Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Influenza A virus-infection differentially affects conventional and regulatory T cell repertoire

ABSTRACT: To investigate the impact of Influenza A virus (IAV) infection on thymic development of mouse regulatory T cells (Tregs) and conventional T cells (Tconv), the TCR repertoire of thymic Tregs and Tconv of IAV-infected and control mice was analyzed.

ORGANISM(S): Mus musculus

PROVIDER: GSE139499 | GEO | 2021/09/09

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

ATAC-seq of FOXP3+ CD4SP regulatory T cells and their FOXP3- CD4SP conventional counterparts from the human thymus

Project description:Characterisation of genome-wide chromatin accessibility (tn5-digested, nucleosome depleted) from thymic Treg and thymic Tconv and to obtain the gene regulatory networks defining thymic Tregs in humans.

2024-10-14 | E-MTAB-11220 | biostudies-arrayexpress

Influenza A virus-infection differentially affects conventional and regulatory T cell repertoire

Project description:Influenza A virus-infection differentially affects conventional and regulatory T cell repertoire

| PRJNA579988 | ENA

Immune Gene Expression Profile of Conventional and Regulatory T cells

Project description:Understanding human regulatory T cells (Tregs) heterogeneity may identify markers of disease pathogenesis and facilitate the development of optimized cellular therapeutics. To better elucidate human Treg subsets, we conducted direct transcriptional profiling of CD4+FOXP3+Helios+ thymic-derived Treg (tTreg) and CD4+FOXP3+Helios- peripherally-induced Treg (pTreg), followed by comparison to CD4+FOXP3-Helios- T conventional (Tconv) cells. This analysis revealed that the coinhibitory receptor T-cell immunoglobulin and immunoreceptor tyrosine-based inhibitory motif domain (TIGIT) was highly expressed on tTreg. In this study CD4 T cells were stained for the Treg-associated transcription factors FOXP3 and Helios, and subsequently FACS sorted to yield three populations: tTreg (CD4+FOXP3+Helios+), pTreg (CD4+FOXP3+Helios–) and the reference population Tconv (CD4+FOXP3–Helios–). A direct transcriptional profile was obtained from the recovered RNA from the populations defined as tTreg, pTreg, and Tconv.

2015-04-30 | E-GEOD-61834 | biostudies-arrayexpress

Immune Gene Expression Profile of Conventional and Regulatory T cells

2015-04-30 | GSE61834 | GEO

Infant antibody repertoires during the first two years of influenza vaccination

Project description:Proteomic analysis of serum IgG antibody repertoire against influenza vaccine HAs in infant donors vaccinated with Fluzone 2017-18/18-19. Dataset consists of peak-response (days 14 and 21 post-vaccination) serum IgG samples eluted by affinity chromatography against IAV or IBV hemagglutinin and the flow-throughs.

2022-08-01 | MSV000090057 | MassIVE

Influenza A virus replication in primary human kidney cells reveals regulated cell death as a potential factor for acute kidney injury in influenza

Project description:Influenza A virus (IAV) infection can cause the lethal acute respiratory distress syndrome of the lung (ARDS). However, AKI is frequently noticed and correlated to an increased mortality. While the causality for influenza-related ARDS is well established, the pathogenesis of influenza-related AKI is incompletely understood. Yet, in post mortem studies of patients infected with H1N1- or H5N6-type IAV, viral antigen was detected in tubular and glomerular cells. Relevantly, receptors for human and avian IAV are present on human kidney cells, viremia was observed and IAV was repeatedly detected by infectivity- and PCR assays in human urine.

2024-05-22 | PXD030093 | Pride

Influenza A virus preferentially snatches non-coding RNA caps

Project description:Influenza A virus (IAV) lacks the enzyme for adding 5â caps to its RNAs, and thus snatches the 5â ends of host capped RNAs to prime transcription. Neither the preference of the host RNA sequences snatched, nor the effect of âcap-snatchingâ on host processes has been completely defined. Previous studies of influenza cap-snatching used poly(A)-selected RNA from infected cells or relied solely on annotated host protein-coding genes to define host mRNAs selected by the virus. To examine the substrate-product relationship between all host RNAs, including non-coding RNAs, and viral RNAs, we used an unbiased approach to identify the host and viral capped RNAs from IAV-infected cells. We demonstrate that IAV predominantly snatches caps from non-coding host RNAs, particularly U1 and U2 small nuclear RNAs (snRNAs). Because snRNAs regulate host mRNA processing, cap-snatching of snRNAs may constitute a means by which IAV hijacks host cell metabolism. examine caps snatched by influenza virus A

2015-11-23 | E-GEOD-67493 | biostudies-arrayexpress

GATM PPMO knockdown affects host response to H1N1 influenza infection in mouse lungs

Project description:The GATM gene shows strong transcriptional regulation during influenza infection and increased GATM transcription correlates with peak viral titers in the lung. In order to investigate an in vivo role for GATM during influenza infection, we created a peptide-conjugated phosphorodiamidate morpholino oligomer (PPMO) to disrupt GATM translation. In our study, mice received a GATM-specific PPMO or scrambled PPMO once per day for two days, and then were subsequently infected with an H1N1 (A/California/04/2009) strain of IAV or PBS as a mock infection control. Mouse lungs were harvested three days post-infection and analyzed via RNA sequencing.

2021-08-02 | GSE181246 | GEO

Alpha/Beta Mouse TCR Sequencing of CD4+ thymic regulatory and conventional T-cells

Project description:Thymic regulatory T cells (tTreg) are critical in maintenance of normal T cell immunity and tolerance. The role of TCR in tTreg cell selection remains incompletely understood. Here we assessed TCRa and TCRb sequences of tTreg and conventional thymic CD4+ T (Tconv) cells by high throughput sequencing. We identified ab TCR sequences that were unique to either tTreg or Tconv cells and found that these were distinct as recognized by machine learning (ML) algorithm and by preferentially used amino acid trimers in ab CDR3 of tTreg cells. In addition, a proportion of ab TCR sequences expressed by tTreg were also found in Tconv cells, and ML classified the great majority of these shared ab TCR sequences as characteristic of Tconv and not tTreg cells. These findings identify two populations of tTreg, one in which Treg fate is associated with unique properties of the TCR, and another with TCR properties characteristic of Tconv cells for which tTreg fate is determined by factors beyond TCR sequence.

2020-01-10 | GSE143291 | GEO

Tconv and Treg gene expression and repertoire analysis of ZAC mice

Project description:Autoimmune diseases, such as rheumatoid arthritis (RA), are believed to be caused, in part, by a defect in the selection of T cells. Developing T cells undergo a process known as “negative selection” where self-derived proteins are presented to the cells; those that react to the self-peptides are either eliminated, or develop into regulatory T cells (Tregs), which suppress immune responses by conventional T cells (Tconvs). In order to study self-reactive T cell receptors (TCRs), and their likely target antigens, we performed single cell repertoire analysis in ZAC mice with impaired TCR signaling. We sequenced TCR and transcriptomes of Treg and Tconv single cells from arthritic ZAC mice spleen (n=2). We discovered a cluster of pro-inflammatory Th17 Tconv cells in ZAC spleens that was absent in WT mice. ZAC Th17 TCRs showed clonal expansion. Different mice had different TCR sequences, which is expected.

2022-11-22 | GSE180015 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data