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Single-Cell transcriptomes and BCR sequences of IgG+ murine memory B cells from Bone Marrow and Spleen

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Mus musculus

PROVIDER: GSE140133 | GEO | 2020/04/24

REPOSITORIES: GEO

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Single-Cell transcriptomes and BCR sequences of IgG+ murine memory B cells from Bone Marrow and Spleen

Project description:Single-Cell transcriptomes and BCR sequences of IgG+ murine memory B cells from Bone Marrow and Spleen

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Single-Cell transcriptomes and immune profiling of IgG+ murine memory B cells from Bone Marrow and Spleen.

Project description:Single cell sequencing for analysis gene of gene transcription and Ig repertoires of isotype-switched IgG-expressing memory B cells for paired analysis with Ig repertoires in the same cells. Aim of the study was to explore heterogeneity of isotype-switched memory B cells within and between spleen and bone marrow compartments.

2020-04-24 | GSE139836 | GEO

Single-cell transcriptomes of murine bone marrow stromal cells reveal niche-associated heterogeneity.

Project description:Bone marrow (BM) stromal cells are important in the development and maintenance of cells of the immune system. Using single cell RNA sequencing, we here explore the functional and phenotypic heterogeneity of individual transcriptomes of 1167 murine BM mesenchymal stromal cells. These cells exhibit a tremendous heterogeneity of gene expression, which precludes the identification of defined subpopulations. However, according to the expression of 108 genes involved in the communication of stromal cells with hematopoietic cells, we have identified 14 non-overlapping subpopulations, with distinct cytokine or chemokine gene expression signatures. With respect to the maintenance of subsets of immune memory cells by stromal cells, we identified distinct subpopulations expressing Il7, Il15 and Tnfsf13b. Together, this study provides a comprehensive dissection of the BM stromal heterogeneity at the single cell transcriptome level and provides a basis to understand their lifestyle and their role as organizers of niches for the long-term maintenance of immune cells.

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Discrete populations of isotype-switched memory B lymphocytes are maintained in murine spleen and bone marrow.

Project description:At present, it is not clear how memory B lymphocytes are maintained over time, and whether only as circulating cells or also residing in particular tissues. Here we describe distinct populations of isotype-switched memory B lymphocytes (Bsm) of murine spleen and bone marrow, identified according to individual transcriptional signature and B cell receptor repertoire. A population of marginal zone-like cells is located exclusively in the spleen, while a population of quiescent Bsm is found only in the bone marrow. Three further resident populations, present in spleen and bone marrow, represent transitional and follicular B cells and B1 cells, respectively. A population representing 10-20% of spleen and bone marrow memory B cells is the only one qualifying as circulating. In the bone marrow, all cells individually dock onto VCAM1+ stromal cells and, reminiscent of resident memory T and plasma cells, are void of activation, proliferation and mobility.

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Single-Cell transcriptomes and immune profiling of IgG+ murine memory B cells from Bone Marrow and Spleen.

Project description:Single-Cell transcriptomes and immune profiling of IgG+ murine memory B cells from Bone Marrow and Spleen.

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Characterization of murine macrophages from bone marrow, spleen and peritoneum.

Project description:Macrophages have heterogeneous phenotypes and complex functions within both innate and adaptive immune responses. To date, most experimental studies have been performed on macrophages derived from bone marrow, spleen and peritoneum. However, differences among macrophages from these particular sources remain unclear. In this study, the features of murine macrophages from bone marrow, spleen and peritoneum were compared.We found that peritoneal macrophages (PMs) appear to be more mature than bone marrow derived macrophages (BMs) and splenic macrophages (SPMs) based on their morphology and surface molecular characteristics. BMs showed the strongest capacity for both proliferation and phagocytosis among the three populations of macrophage. Under resting conditions, SPMs maintained high levels of pro-inflammatory cytokines expression (IL-6, IL-12 and TNF-?), whereas BMs produced high levels of suppressive cytokines (IL-10 and TGF-?). However, SPMs activated with LPS not only maintained higher levels of (IL-6, IL-12 and TNF-?) than BMs or PMs, but also maintained higher levels of IL-10 and TGF-?.Our results show that BMs, SPMs and PMs are distinct populations with different biological functions, providing clues to guide their further experimental or therapeutic use.

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Gene expression of murine IgG2-memory B cells from spleen and bone marrow

Project description:We compared gene expression profiles of murine IgG2b+ memory B cells isolated from spleen and bone marrow of C57BL/6J mice and mice obtained at a pet shop.

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Non-Hematopoietic and Hematopoietic SIRP? Signaling Differently Regulates Murine B Cell Maturation in Bone Marrow and Spleen.

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Reactivation of IgG-switched memory B cells by BCR-intrinsic signal amplification promotes IgG antibody production.

Project description:Secondary antibody responses are marked by faster kinetics, improved antibody affinity and a switch from IgM to other immunoglobulin isotypes, most notably IgG, compared with primary responses. These changes protect from reinfection and represent the principle of most vaccination strategies. Yet, the molecular mechanisms that underlie B-cell memory responses are unclear. Here we show, by inactivating the immunoglobulin tail tyrosine (ITT) signalling motif of membrane-bound IgG1 in the mouse, that the ITT facilitates maintenance and reactivation of IgG-switched memory B cells in vivo. The ITT motif equips IgG-switched cells with enhanced BCR signalling capacity, which supports their competitiveness in secondary immune reactions and drives the formation of IgG-secreting plasma cells even in the absence of T-cell help. Our results demonstrate that ITT signalling promotes the vigorous production of IgG antibodies and thus provide a molecular basis for humoral immunological memory.

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CD69<sup>+</sup> memory T lymphocytes of the bone marrow and spleen express the signature transcripts of tissue-resident memory T lymphocytes.

Project description:It is a matter of current debate whether the bone marrow is a hub for circulating memory T lymphocytes and/or the home of resident memory T lymphocytes. Here we demonstrate for CD69+ murine CD8+ , and CD69+ murine and human CD4+ memory T lymphocytes of the bone marrow, making up between 30 and 60% of bone marrow memory T lymphocytes, that they express the gene expression signature of tissue-resident memory T lymphocytes. This suggests that a substantial proportion of bone marrow memory T lymphocytes are resident. It adds to previous evidence that bone marrow memory T cells are resting in terms of mobility and proliferation, and maintain exclusive long-term memory to distinct, systemic antigens.

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