Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNAseq anaysis of hippocamal GAD2 cells

ABSTRACT: Purpose: To identify gene expression in GAD2 neurons of the hippocsmpus Method: Translating Ribosome Affinity Purification (TRAP) to isolate RNA from GAD2+ cells, cDNA synthesis and next generation RNAseq using Illumina Nextseq sequencer. Results: GAD2 had ~8000 of enriced genes as compared to bulk mRNA

ORGANISM(S): Mus musculus

PROVIDER: GSE141483 | GEO | 2023/02/11

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

RNAseq anaysis of hippocamal cck cells of SMARCA3 cKO mice

Project description:Purpose: To identify gene expression changes in CCK neurons of hippocsmpus of SMARCA3 cKO mice Method: Translating Ribosome Affinity Purification (TRAP) to isolate RNA from CCK+ cells and, cDNA synthesis and next generation RNAseq using Illumina Nextseq sequencer. Results: Biostatistical analysis identified 1378 genes that were altered by SMARCA3 cKO.

2023-02-11 | GSE141481 | GEO

RNAseq anaysis of hippocamal parvalbumin-positive interneurons in mice exposed to chronic social defat stress

Project description:Purpose: To identify genes differentially expressed in parvalbumin-positive (PV) interneurons of the hippocampus in non-defetaed (CTRL), stress-susceptible and stress-resilient mice. Method: Translating Ribosome Affinity Purification (TRAP) to isolate RNA from PV+ cells, cDNA synthesis and next generation RNAseq using Illumina Nextseq 500 sequencer. Results: RNA-seq revealed 458 DEGs between non-defeated and resilient mice, 1976 DEGs in non-defeated and susceptible mice, and 3475 DEGs in resilient and susceptible mice.

2021-09-14 | GSE184027 | GEO

RNAseq anaysis of ventral striatum cholinergic cells of p11 cKO mice

Project description:Purpose: To identify gene expression changes in cholinergic neurons of the ventral striatum of p11 cKO mice Method: Translating Ribosome Affinity Purification (TRAP) to isolate RNA from ChAT+ cells and, cDNA synthesisi and next generation RNAseq using Illumina Hiseq sequencer. Results: Biostatistical analysis identified 113 genes that were altered by p11. 59 genes were up-regulated and 54 down-regulated.

2018-11-01 | GSE106389 | GEO

Hypoxia-induced translational profiles of murine fetal liver derived macrophages (FLDM)

Project description:We report changes in total and translated poly(A) RNA in mouse fetal liver derived macrophages after exposure to hypoxia. We employed translating ribosome affinity purification (TRAP) to isolate polysomal RNA from mouse fetal liver derived macrophages after exposure to hypoxia.

2023-09-07 | GSE196966 | GEO

Hypoxia-induced translational profiles of murine bone marrow derived macrophages (BMDM)

Project description:We report changes in total and translated poly(A) RNA in mouse bone marrow derived macrophages after exposure to hypoxia. We employed translating ribosome affinity purification (TRAP) to isolate polysomal RNA from mouse bone marrow derived macrophages after exposure to hypoxia

2023-09-07 | GSE196517 | GEO

Gene expression in Sox9 progenitors in the developing mouse lung

Project description:This submission consists of two experiments utilizing translating ribosome affinity purification-sequencing (TRAP-seq). The first uses TRAP-seq to isolate transcripts from Sox9 positive progenitors at E14 and an E17 lung and compares it to non-immunoprecipitated whole lung homogenate control. The second compares TRAP-seq isolated transcripts from Sox9-wild type progenitors versus mutant progenitors. Recombination was induced at E13 and cells were isolated at E16.

2018-03-01 | GSE101582 | GEO

RNAseq anaysis of entorhinal cortex layer II neurons upon Ptbp1 modulation

Project description:Purpose: To identify gene expression changes in entorhinal cortex layer II (ECII) neurons upon Ptbp1 modulation (silencing and overexpression) Method: bacterial artificial chromosome - Translating Ribosome Affinity Purification (bacTRAP) to isolate actively translated mRNA in ECII neurons, 2 weeks after stereotaxic injection of an AAV1 vector in the EC of ECII-bacTRAP mice; followed by RNAseq. Note: Ptbp1 was significantly overexpressed in the overexpression experiment, but no silencing was achieved with the silencing vector, probably because of tight control of Ptbp1 expression

2020-05-29 | GSE151356 | GEO

L1CAM dependent transcriptional programs in brain metastatic cells

Project description:L1CAM dependent gene expression programs in vitro and ex vivo were analyzed by translating ribosome affinity purification and sequencing (TRAP-Seq).

2018-07-23 | GSE82281 | GEO

TRAP-seq of VMH Nr5a1 neurons

Project description:To identify transcripts enriched in ventomedial hypothalamus (VMH) neurons, we used translating affinity ribosome purification combined with RNA-seq (TRAP-seq) from Nr5a1-Cre mice.

2021-05-19 | GSE172206 | GEO

TRAP-seq of VMH Lepr neurons

Project description:To identify transcripts enriched in ventomedial hypothalamus (VMH) Lepr neurons, we used translating affinity ribosome purification combined with RNA-seq (TRAP-seq) from Lepr-Cre mice.

2021-05-19 | GSE172200 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data