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Zscan4 mediates ubiquitination and degradation of co-repressor complex to promote chromatin accessibility in two-cell embryos and ESCs

ABSTRACT: Zscan4 regulates chromatin accessibility and 2C in early embryos or 2C-like state in ESC cultures.

ORGANISM(S): Mus musculus

PROVIDER: GSE141490 | GEO | 2022/08/16

REPOSITORIES: GEO

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Zscan4 mediates ubiquitination and degradation of co-repressor complex to promote chromatin accessibility in two-cell embryos and ESCs

Project description:Zscan4 regulates chromatin accessibility and 2C in early embryos or 2C-like state in ESC cultures.

2022-08-16 | GSE141489 | GEO

Zscan4 binds nucleosomal microsatellite DNA and protects mouse two-cell embryos from DNA damage [ChIP-seq]

Project description:Genome-wide binding analysis of Zscan4 showed sequence-specific occupancy at (CA)n microsatellite repeats in 2C-like cells. Genome-wide occupancy profiling of FACT subunit SSRP1 in curaxin-treated mouse ESC demonstrated re-localization from transcription start sites to Zscan4 binding sites.

2020-03-22 | GSE140619 | GEO

Zscan4 binds nucleosomal microsatellite DNA and protects mouse two-cell embryos from DNA damage [RNA-seq]

Project description:2C-like cells or pluripotent cells were isolated from Zprom::GFP and Zprom::GFP-Zscan4 mouse ESC reporter lines to measure changes in gene expression.

2020-03-22 | GSE140615 | GEO

Zscan4 mediates ubiquitination and degradation of co-repressor complex to promote chromatin accessibility in two-cell embryos and ESCs

Project description:Zscan4 mediates ubiquitination and degradation of co-repressor complex to promote chromatin accessibility in two-cell embryos and ESCs

| PRJNA593596 | ENA

Zscan4 mediates ubiquitination and degradation of co-repressor complex to promote chromatin accessibility in two-cell embryos and ESCs

Project description:Zscan4 mediates ubiquitination and degradation of co-repressor complex to promote chromatin accessibility in two-cell embryos and ESCs

| PRJNA593599 | ENA

MERVL/Zscan4 network activation results in transient genome-wide DNA demethylation of mESCs

Project description:Several previous studies have noted a rare sub-population of murine Embryonic Stem Cells (mESCs) which seem to express genes otherwise specific to the 2-cell stage of embryonic development. Here we sorted single cells from a MERVL/Zscan4 reporter line to investigate the continuum of expression as cells go from the standard state to this 2C-like state.

2016-09-30 | E-MTAB-5058 | biostudies-arrayexpress

Retinoic acid specifically enhances embryonic stem cell metastate marked by Zscan4

Project description:Pluripotency confers Embryonic Stem Cells (ESCs) the ability to differentiate in ectoderm, endoderm, and mesoderm derivatives, producing the majority of cell types. Although the majority of ESCs divide without losing pluripotency, it has become evident that ESCs culture consists of multiple cell populations with different degrees of potency that are spontaneously induced in regular ESC culture conditions. Zscan4, a key pluripotency factor, marks ESC subpopulation that is referred to as high-level of pluripotency metastate. Here, we report that in ESC cultures treated with retinoic acid (RA), Zscan4 ESCs metastate is strongly enhanced. In particular, we found that induction of Zscan4 metastate is mediated via RA receptors (RAR-alpha, RAR-beta, and RAR-gamma), and it is dependent on phosphoinositide-3-kinase (PI3K) signaling. Remarkably, Zscan4 metastate induced by RA lacks canonical pluripotency genes Oct3/4 and Nanog but retained both self-renewal and pluripotency capabilities. Finally we demonstrated that the conditional ablation of Zscan4 subpopulation is dispensable for both endoderm and mesoderm but is required for ectoderm lineage. In conclusion, our research provides new insights about the role of RA signaling during ESCs high pluripotency metastate fluctuation.

2017-02-13 | GSE75977 | GEO

Zscan4 mediates transient remodeling and transcriptional burst of heterochromatin in mouse embryonic stem cells

Project description:This SuperSeries is composed of the SubSeries listed below. Repetitive sequences such as telomeres, centromeres, and retrotransposons are packed in permanently-condensed and transcriptionally-silenced heterochromatin, whose maintenance is critical for the genome stability. We have recently found that mouse ES cells occasionally go through a unique cell state marked by the transient expression of Zscan4, which plays a key role in long-term genome stability. Here we report our unexpected and counterintuitive finding that the Zscan4+ state is accompanied by a Zscan4-mediated rapid activation and repression of heterochromatin: the burst of transcription from heterochromatin coincided with its transient histone hyperacetylation and DNA demethylation, the clustering of pericentromeric heterochromatin in the perinucleolar region, and the accumulation of chromatin remodeling complexes for both activation (HATs, SWI/SNF) and repression (HDACs, LSD1/KDM1A, NuRD) on heterochromatin. Overall, these findings suggest that ES cells maintain their extraordinary genome stability and immortality by transiently taking extreme measures – a usually forbidden transition to the activated state of heterochromatin. Refer to individual Series

2015-09-10 | E-GEOD-51682 | biostudies-arrayexpress

CHIP-seq analysis about Histones and Zscan4 after Zscan4 overexpression

Project description:Transcription factor Zscan4 was newly discovered to be a marker of the intermediate state of 2-cell like cells . In addition, the WGCNA discovered a strong positive correlation between the expression of Zscan4 and that of MT2/MERVL .Hence, we examined the possibility for Zscan4 as an activator of MT2/MERVL.

2019-07-08 | GSE125238 | GEO

CTCF is a Barrier for Totipotent-like Reprogramming [END-seq]

Project description:Totipotent cells have the ability of generating embryonic and extra-embryonic tissues. Interestingly, a rare population of cells with totipotent-like potential was identified within ESC cultures. These cells, known as 2 cell (2C)-like cells, arise from ESC and display similar features to those found in the totipotent 2 cell embryo. However, the molecular determinants of 2C like conversion have not been completely elucidated. Here, we show that CTCF is a barrier for 2C-like reprogramming. Indeed, forced conversion to a 2C-like state by DUX expression was associated with DNA damage at a subset of CTCF binding sites. Endogenous or DUX-induced 2C-like ESC showed decreased CTCF enrichment at known binding sites, suggesting that acquisition of a totipotent-like state is associated with a highly dynamic chromatin architecture. Accordingly, depletion of CTCF in ESC efficiently promoted spontaneous and asynchronous conversion to a totipotent-like state. This phenotypic reprogramming was reversible upon restoration of CTCF levels. Furthermore, we showed that transcriptional activation of the ZSCAN4 cluster was necessary for successful 2C-like reprogramming. In summary, we revealed the intimate relation between CTCF and totipotent-like reprogramming.

2021-06-07 | GSE165161 | GEO

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