Amino acid metabolism regulated by DNA Methylation in Mycobacterium bovis BCG str. Tokyo 172 induced by vitamins

ABSTRACT: Vitamins are organic compounds and essential nutrients required by host organism. Recent studies have shown that vitamin B1(VB1) and vitamin C (Vc) could inhibit Mycobacterium tuberculosis growth. However, the precise role of VB1 and Vc in M.tuberculosis are still not well understand. Therefore, in this study, the transcriptional, metabolic and methylation profiles of VB1 and VC treated Mycobacterium. bovis BCG were analyzed by RNA-sequencing, LC-MS (Liquid chromatography coupled to mass spectrometry) and SMRT. The RNA-seq show that gene encoding cysteine synthase A was significantly up-regulated under both vitamins treatment, metabolic study show that most of amino acids decreased in VB1 treated BCG, in constrast, most of amino acids increased in VC treated samples. Methylome data show that m4c modificatiion partically is present in VC treated BCG and the expression of most genes harboring m4c methylation modificaiton increased. In all, these data imply that potentially increased concentration of cysteine might lead to BCG growth inhibition induced by VB1 and VC duing to produce superoxide which could cause DNA damage via Fetions reacition. The m4c methylation modification could promote gene transcription to some extent. In all, this study will benefit for us to futher understand the roles of VB1 and VC which could be used as potential anti-tuberculosis drugs.

ORGANISM(S): Mycobacterium tuberculosis variant bovis

PROVIDER: GSE141513 | GEO | 2020/01/16

REPOSITORIES: GEO