Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Expression data from adult myocardium of ToF-PVR patients undergoing pulmonary valve replacement

ABSTRACT: Adult right ventricle from Tetralogy of Fallot patients undergoing pulmonary valve replacement vs right ventricle myocardium from unused donor hearts we used Microarray to determine whether there were differences in gene expression between these groups

ORGANISM(S): Homo sapiens

PROVIDER: GSE141955 | GEO | 2020/06/22

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Investigation of TOF patient heart specimens

Project description:The protein BBLN with largely unknown function was identified in human right ventricular heart specimens from patients undergoing repair surgery for Tetralogy of Fallot (TOF). Proteins from heart specimens were solubilized and subjected to immuno-affinity enrichment with anti-CAMK2D-specific antibodies. Co-enriched proteins were separated by SDS-PAGE, and the protein band in the range of 8-14 kDa was cut and subjected to identification by nano-LC-ESI-MS/MS.

2023-09-18 | PXD044695 | Pride

Noncoding RNA expression in myocardium from infants with tetralogy of Fallot

Project description:This SuperSeries is composed of the following subset Series: GSE35490: Noncoding RNA expression in myocardium from infants with tetralogy of Fallot [miRNA profiling] GSE35776: Noncoding RNA expression in myocardium from infants with tetralogy of Fallot [mRNA profiling] Refer to individual Series

2012-07-11 | E-GEOD-35781 | biostudies-arrayexpress

Transcriptome sequencing in patients with Tetralogy of Fallot and healthy unaffected individuals [LncRNA/mRNA]

Project description:The right ventricular tissues of 5 children with tetralogy of Fallot and 5 healthy unaffected individuals were collected and sequenced in full transcriptome. Mechanism of tetralogy of fallot and cerna regulatory network were analyzed.

2023-03-13 | GSE217772 | GEO

Transcriptome sequencing in patients with Tetralogy of Fallot and healthy unaffected individuals [miRNA]

2023-03-13 | GSE217771 | GEO

mRNA expression profiling in patients with Tetralogy of Fallot and healthy unaffected individuals

Project description:Right ventricular mRNA profiles from 22 patients with Tetralogy of Fallot (TOF) and mRNA-seq profiles from the left and right ventricle (LV and RV, respectively) of 4 healthy unaffected individuals (NH) were generated. The total RNA was isolated from the 30 human heart samples using TRIzol. mRNAs were isolated from total RNA and prepared for sequencing using Illumina Kit RS-100-0801 according to the manufacturer's protocol (Preparing Samples for Sequencing of mRNA Sept 2008). The sequencing libraries were generated using a non-strand-specific library construction method. The purified DNA fragments were used directly for cluster generation, and 36 cycles of single-end read sequencing were performed using the Illumina Genome Analyzer. Sequencing reads were extracted from the image files using the open source Firecrest and Bustard applications (Illumina Genome Analyzer pipeline 1.3.2, 1.4.0 and 1.5.0). Right ventricular mRNA-seq profiles from 22 patients with Tetralogy of Fallot and mRNA-seq profiles from the left and right ventricle of 4 healthy unaffected individuals. Supplementary processed data files: GSE36761_gene_expression_levels.txt: Gene expression profiling data for all samples. GSE36761_gene_expression_levels_normalized.txt: Normalized gene expression profiling data for all samples. GSE36761_transcript_expression_levels.txt: Transcript expression profiling data for all samples. GSE36761_transcript_expression_levels_normalized.txt: Normalized transcript expression profiling data for all samples. Genome build: hg18

2014-02-04 | E-GEOD-36761 | biostudies-arrayexpress

Gene expression in cardiac tissues from infants with idiopathic conotruncal defects

Project description:Tetralogy of Fallot (TOF) is the most commonly seen type of conotruncal congenital heart defect. Treatment of these patients has evolved dramatically in the last few decades, yet a genetic explanation for the failure of cardiac development is lacking for the majority of children with TOF. Our goal was to examine genome wide gene expression to characterize expression patterns in cardiovascular tissue (right ventricle, pulmonary valve and pulmonary artery) obtained at the time of reconstructive surgery from 19 children with tetralogy of Fallot. We employed genome wide gene expression microarrays to characterize cardiovascular tissue (right ventricle, pulmonary valve and pulmonary artery) obtained at the time of reconstructive surgery from 19 children with tetralogy of Fallot (16 idiopathic and 3 with 22q11.2 deletions) and compared gene expression patterns to normally developing subjects. We detected a signal from approximately 26,000 probes (ranging from 35% to 49% of array probes in the three tissues), reflecting expression from about half of all genes. More than 1000 genes had a 2-fold change in expression in the right ventricle (RV) of children with TOF compared to RV from matched control infants. Most of these genes were involved in compensatory functions (e.g., hypertrophy, cardiac fibrosis and cardiac dilation). However, two canonical pathways involved in spatial and temporal cell differentiation (WNT, p = 0.017 and Notch, p = 0.003) appeared to be generally suppressed. The suppression of developmental networks may be a remnant of a broad malfunction of regulatory pathways leading to inaccurate boundary formation and improper structural development in the embryonic heart. We suggest that small tissue specific genomic and/or epigenetic fluctuations could be cumulative, leading to regulatory network disruption and failure of proper cardiac development.

2011-01-20 | GSE26125 | GEO

Whole genome microarray gene expression profiling of right ventricular heart specimens from pediatric patients with Tetralogy of Fallot (TOF)

Project description:Tetralogy of Fallot (TOF) is the most common cyanotic congenital heart defect with a world-wide prevalence of 3 to 4 cases per 10,000 life births. TOF is a congenital heart disease with four major cardiac defects, i.e., ventricular septal defect, overriding aortic root, infundibular stenosis of the pulmonary artery, and right ventricular hypertrophy. Treatment relies on correction surgery in early infancy. This study performed whole genome microarray gene expression profiling of cardiac specimens of the right ventricular outflow tract (RVOT), which were recovered during correction surgery of TOF from 11 pediatric patients diagnosed with TOF cardiac defects.

2023-09-18 | GSE241024 | GEO

Transcription profile in patients with cyanotic Tetralogy of Fallot undergoing corrective surgery using hyperoxic/standard cardiopulmonary bypass

Project description:To determine cardiac transcription profile in cyanotic Tetralogy of Fallot patients subjected to hyperoxic/standard cardiopulmonary bypass, we collected myocardial samples at the end of the ischemic time. The transcriptional profile of the mRNA in these samples was measured with gene array technology Myocardial samples were collected at the end of ischemic time from cyanotic Tetralogy of Fallot patients undergoing corrective surgery using hyperoxic/standard cardiopulmonary bypass

2012-12-06 | E-GEOD-38161 | biostudies-arrayexpress

Transcription profile in patients with cyanotic Tetralogy of Fallot undergoing corrective surgery using controlled reoxygenation cardiopulmonary bypass

Project description:To determine cardiac transcription profile in cyanotic Tetralogy of Fallot patients subjected to conrolled reoxygenation cardiopulmonary bypass, we collected myocardial samples at the end of the ischemic time. The transcriptional profile of the mRNA in these samples was measured with gene array technology Myocardial samples were collected at the end of ischemic time from cyanotic Tetralogy of Fallot patients undergoing corrective surgery using conrolled reoxygenation cardiopulmonary bypass

2012-12-06 | E-GEOD-38162 | biostudies-arrayexpress

Expression data from adult myocardium of ToF-PVR patients undergoing pulmonary valve replacement

Project description:Expression data from adult myocardium of ToF-PVR patients undergoing pulmonary valve replacement

| PRJNA595380 | ENA