Transcriptomics

Dataset Information

0

Chromatin Topology Reorganization and Transcription Repression by PML/RARα in Acute Promyeloid Leukemia (RNA-seq)


ABSTRACT: Acute promyeloid leukemia (APL) is characterized by the oncogenic fusion protein PML/RARα, a major etiological agent in APL. Although PML/RARα is critical, the molecular mechanisms remains largely unknown. Here, using an inducible system, we comprehensively analyzed the 3D genome organization in myloid cells and its reorganizationn after PML/RARα induction, and performed additional analysis in patient-derived APL cells with native PML/RARα. We discovered that PML/RARα mediate extensive chromatin interactions genome-wide. Globally, it redefine the chromatin topology of the in myloid genome toward a more condensed configuration in APL cells; locally, it intrude RNAPII-associated interaction dmains, interrupt myeloid-specific transcription factors binding at enhancers and super-enahncers, and lead to transcriptional repression of genes critical for myeloid differentiation and maturation. Together, our results provide novel insights of a topological framework for PML/RARα’s roles in transforming myeloid into leukemia, likely a general mechanism for oncogenic fusion proteins in cancers.

ORGANISM(S): Homo sapiens

PROVIDER: GSE142198 | GEO | 2019/12/17

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2019-12-05 | GSE137661 | GEO
2019-12-17 | GSE142197 | GEO
2020-08-26 | GSE126720 | GEO
2019-12-18 | PXD008761 | Pride
2021-04-01 | GSE152397 | GEO
2015-09-04 | E-GEOD-54038 | biostudies-arrayexpress
2022-07-05 | GSE207432 | GEO
2024-07-30 | GSE232294 | GEO
2015-09-04 | GSE54038 | GEO
2020-04-10 | GSE137103 | GEO