Genomics

Dataset Information

0

Histone H3 Lysine 4 mono-, di- and trimethyl and CTCF in CD4+CD25+CD45RA+ regulatory and conventional CD4+CD25- T-cells


ABSTRACT: Analysis of Histone H3 Lysine 4 mono-, di- and trimethyl and the boundary protein CTCF in CD4+CD25+CD45RA+ regulatory T-cells and conventional CD4+CD25- T-cells. To investigate regulatory functions or potential new transcription start sites in Treg and Tconv cells, we investigated the associated histone modifications. Mono- and dimethylation of histone 3 lysin 4 (H3K4) were previously shown to mark enhancer regions, whereas H3K4 trimethylation generally associates with transcription start sites. At imprinted loci, binding of the insulator protein CTCF, which restricts or directs enhancer-promoter interactions, is often regulated by DNA-methylation. Therefore we performed ChIP-on-chip experiments (chromatin immunoprecipitation followed by microarray hybridization; samples were amplified with ligation mediated PCR [see label protocol for the procedure] prior to labeling) for mono- di- and trimethylation of histone 3 lysin 4 and of CTCF in expanded Treg and Tconv cells. Keywords: ChIP-on-chip

ORGANISM(S): Homo sapiens

PROVIDER: GSE14234 | GEO | 2009/05/20

SECONDARY ACCESSION(S): PRJNA114451

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2010-05-19 | E-GEOD-14234 | biostudies-arrayexpress
2010-05-19 | E-GEOD-14281 | biostudies-arrayexpress
2021-05-04 | GSE145399 | GEO
2018-03-15 | GSE111901 | GEO
2010-12-07 | GSE11657 | GEO
2008-12-19 | E-GEOD-6813 | biostudies-arrayexpress
| PRJNA114451 | ENA
2014-04-01 | E-GEOD-43119 | biostudies-arrayexpress
2010-05-18 | E-GEOD-14233 | biostudies-arrayexpress
2013-12-17 | E-GEOD-50417 | biostudies-arrayexpress