Project description:Monocyte-derived DCs (moDCs) have been implicated in the pathogenesis of autoimmunity, but the molecular pathways determining the differentiation potential of these cells remain unclear. Here, we report that microRNA-148a (miR-148a) serves as a critical regulator for moDC differentiation. First, miR-148a deficiency impaired the moDC development in vitro and in vivo. A mechanism study showed that MAFB, a transcription factor that hampers moDC differentiation, was a direct target of miR-148a. In addition, a promoter study identified that miR-148a could be transcriptionally induced by PU.1, which is crucial for moDC generation. miR-148a ablation eliminated the inhibition of PU.1 on MAFB. Furthermore, we found that miR-148a increased in monocytes from patients with psoriasis, and miR-148a deficiency or intradermal injection of antagomir-148a immensely alleviated the development of psoriasis-like symptoms in a psoriasis-like mouse model. Therefore, these results identify a pivotal role for the PU.1-miR-148a-MAFB circuit in moDC differentiation and suggest a potential therapeutic avenue for autoimmunity.

| S-EPMC7205423 | biostudies-literature

Runx3 regulated genes in splenic CD4+ dendritic cells

Project description:This SuperSeries is composed of the SubSeries listed below.

2014-06-22 | GSE48591 | GEO

The role of microRNA-regulating energy metabolism and pollen wall development in male sterility in cotton

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2013-07-28 | E-GEOD-43532 | biostudies-arrayexpress

Alteration of mRNA and microRNA expression profiles in rat muscular type vasculature in early postnatal development

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2015-04-30 | E-GEOD-60963 | biostudies-arrayexpress

Divergent principles specifying inflammatory versus antiviral transcriptional programs in dendritic cells

Project description:This SuperSeries is composed of the SubSeries listed below.