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Unknown,Transcriptomics,Genomics,Proteomics

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Runx3 regulated genes in splenic CD4+ dendritic cells

ABSTRACT: This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

ORGANISM(S): Mus musculus

SUBMITTER: Yoram Groner

PROVIDER: E-GEOD-48591 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Transcriptional reprogramming of CD11b+Esam(hi) dendritic cell identity and function by loss of Runx3.

Dicken Joseph J Mildner Alexander A Leshkowitz Dena D Touw Ivo P IP Hantisteanu Shay S Jung Steffen S Groner Yoram Y

PloS one 20131015 10

Classical dendritic cells (cDC) are specialized antigen-presenting cells mediating immunity and tolerance. cDC cell-lineage decisions are largely controlled by transcriptional factor regulatory cascades. Using an in vivo cell-specific targeting of Runx3 at various stages of DC lineage development we show that Runx3 is required for cell-identity, homeostasis and function of splenic Esam(hi) DC. Ablation of Runx3 in DC progenitors led to a substantial decrease in splenic CD4(+)/CD11b(+) DC. Combin ...[more]

PMID: 24204843

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The affect of specific ablation of Runx3 from Esam splenic dendritic cells

Project description:Esam/CD4+ dendritic cells are part of the innate immunity essential for priming and activating of CD4+ T cells To identify Runx3 responsive genes Esam dendritic cells were freshly sorted from macs enriched splenic DCs taken from 6 weeks old mice. Four samples from four mice were sorted and analyzed where in each littermates pair consisted of a control and Runx3 conditional KO. Mice lacking Runx3 specifically in the DC compartment were produced by crossing Runx3fl/fl mice onto CD11c-Cre mice. This mating scheme generated Runx3fl/fl/CD11c:Cre (CD11c-DC-Runx3Δ) mice.

2014-06-22 | E-GEOD-48590 | biostudies-arrayexpress

Runx3 function in CD4+ splenic dendritic cells

Project description:CD4+ dendritic cells are part of the innate immunity essential for priming and activating of CD4+ T cells To identify Runx3 responsive genes CD4+ dendritic cells were sorted from freshly isolated macs enriched splenic DCs taken from 6 weeks old mice. Six samples from six mice were sorted and analyzed where in each littermates pair consisted of a control and Runx3 KO.

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Notch2-dependent classical dendritic cells orchestrate intestinal immunity against attaching and effacing bacterial pathogens

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2013-06-01 | E-GEOD-45698 | biostudies-arrayexpress

Microarray analysis of WT ESAM+ and ESAM- cDC subsets

Project description:Defense against attaching and effacing (A/E) bacteria requires the sequential generation of IL-23 and IL-22 to induce protective mucosal responses. While the critical source of IL-22 has been identified as CD4+ and Nkp46+ innate lymphoid cells (ILCs), the precise source of IL-23 is unclear. Here, we use genetic techniques to deplete specific classical dendritic cell (cDC) subsets and analyze immunity to the A/E pathogen Citrobacter rodentium. We find that Zbtb46+ cDCs, and specifically Notch2-dependent intestinal CD11b+ cDCs, but not Batf3-dependent CD103+ cDCs, are required for IL-23 production and immunity against C. rodentium. Notch2 controls cDC differentiation at a terminal step mediated by lymphotoxin signaling. Importantly, these results provide the first demonstration of a non-redundant function of CD11b+ cDCs in vivo. Analysis of differentially expressed genes in ESAM+ and ESAM- CD11b+ and DEC205+ splenic classical DC subsets. Splenocytes were harvested from WT C57Bl/6 or WT Cx3cr1-gfp mice and cDC subsets sorted to >95% purity on the FACSAriaII.

2013-06-01 | E-GEOD-45679 | biostudies-arrayexpress

Runx3 function in CD8+ splenic T cells

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Zbtb46 expression distinguishes classical dendritic cells and their committed progenitors from other immune lineages

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2013-11-18 | E-GEOD-50121 | biostudies-arrayexpress

Genome-wide maps of Runx3 bound regions in CD4 splenic dendritic cells.

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The transcription program of Runx3 in natural killer cells and CD8+ T cells

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2013-11-18 | E-GEOD-50131 | biostudies-arrayexpress

Microarray analysis of Zbtb46 KO CD4+ splenic DCs and bone marrow erythroid progenitors

Project description:Zbtb46 represses G-CSFR and LifR in cDCs Zbtb46 does not significantly affect gene expression in erythroid progenitors WT, Het, and KO cells were sorted from BM or spleen and analyzed. Pre MegE cells were sorted as CD117+ CD150+ CD105-CD41-CD16/32-. Pre CFU-E cells were sorted as CD117+ CD150+ CD105lo CD41- CD16/32-. CFU-E cells were sorted as CD117+ CD150- CD105+ Cd41- CD16/32-. Splenic CD4+ DCs were sorted as B220- CD11c+ MHCII+ CD8- CD172+ CD11b+ CD4+.

2012-05-20 | E-GEOD-37028 | biostudies-arrayexpress

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