Methylation profiling

Dataset Information

0

DNMTs Play an Important Role in Maintaining the Pluripotency of Leukemia Inhibitory Factor Dependent Embryonic Stem Cells


ABSTRACT: Naïve pluripotency can be maintained in 2i/LIF supplements, which primarily affect canonical WNT, FGF/ERK, and JAK/STAT3 signaling. However, whether one of these tripartite supplements alone is sufficient to maintain naïve self-renewal remain unclear. Here we show that LIF alone medium is sufficient for adaptation of 2i/L-ESCs to ESCs with hypermethylated state (L-ESCs). Global transcriptomic analysis show that L-ESCs are close to 2i/L-ESCs and in a stable state between naïve and primed pluripotency. Notably, our results demonstrate that DNA methyltransferases (DNMTs) play an important role for LIF-dependent mouse ESCs adaptation and self-renew. LIF-dependent ESCs adaptation efficiency is significantly increased in serum treatment and reduced in Dnmt3a or Dnmt3l knockout ESCs. Importantly, unlike epiblast stem cells, L-ESCs contribute to somatic tissues and germ cells in chimaeras. L-ESCs cultured in such a simple conditions in this study would provide a more conducive platform to clarify the molecular mechanism of ESCs in vitro culture.

ORGANISM(S): Mus musculus

PROVIDER: GSE142799 | GEO | 2021/01/25

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2018-11-07 | PXD009217 | Pride
2021-02-10 | PXD018694 | Pride
2024-03-03 | GSE226316 | GEO
2013-01-18 | E-GEOD-42580 | biostudies-arrayexpress
2015-09-14 | E-GEOD-70546 | biostudies-arrayexpress
2018-11-24 | GSE101675 | GEO
2015-07-01 | E-GEOD-69055 | biostudies-arrayexpress
2017-07-27 | GSE97995 | GEO
2021-07-21 | GSE168423 | GEO
2021-02-10 | MTBLS301 | MetaboLights