Measuring the dependence of the yeast heat shock response on intracellular pH during stress
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ABSTRACT: S. cerevisiae cells acidify when they experience stressful temperatures. To determine whether this heat-shock associated acidification is important for the cellular response, we manipulated intracellular pH, heat shocked cells, and sequenced the transcriptome.
Project description:S. cerevisiae cells acidify when they experience stressful temperatures. In addition, newly-translated proteins are thought to misfold, triggering the heat shock response. To determine whether heat-shock associated acidification and translation state are important for the cellular response, we manipulated intracellular pH, blocked translation, heat shocked cells, and sequenced the transcriptome.
Project description:Analysis of mammalian cell responses to digital microfluidic manipulation. BA/F3 cells were manipulated on devices with 15 min continuous actuation at 400Vpp at 1 kHz or 18 kHz and compared to untreated control cells and cells heat shocked at 42C, 47C or 52C.
Project description:Analysis of mammalian cell responses to digital microfluidic manipulation. BA/F3 cells were manipulated on devices with 15 min continuous actuation at 400Vpp at 1 kHz or 18 kHz and compared to untreated control cells and cells heat shocked at 42C, 47C or 52C. Total RNA isolated from Ba/F3 murine cells, untreated or exposed to digital microfludic manipulation or heat shock.
Project description:Transcriptional profiling of heat-shocked worms was compared to non-heat-shocked worms to determine genes that are induced upon heat shock in each species; heat-shock-induced genes in each species were compared
Project description:The blue light receptor WC-2 was shown to be involved in mushroom development in the basidiomycete Schizophyllum commune. When the gene encoding WC-2 was deleted, no mushrooms formed and colony morphology was radial. This phenotype was similar to the wild-type colony grown in the dark. This phenotype could be complemented by transforming the wc-2 deletion strain with a construct encompassing the wc-2 coding sequence under the control of the heat inducible promoter hsp3. A daily heat shock of 1 hour at 42 degrees Celsius resulted in mushroom development and an asymmetrical colony. In this study we performed a genome-wide expression analysis on dikaryons of wild-type (not heat shocked), delta-wc2 (heat shocked or not heat shocked) and the complemented strain delta-wc2 hsp3-wc2 (heat shocked or not heat shocked).
Project description:The blue light receptor WC-2 was shown to be involved in mushroom development in the basidiomycete Schizophyllum commune. When the gene encoding WC-2 was deleted, no mushrooms formed and colony morphology was radial. This phenotype was similar to the wild-type colony grown in the dark. This phenotype could be complemented by transforming the wc-2 deletion strain with a construct encompassing the wc-2 coding sequence under the control of the heat inducible promoter hsp3. A daily heat shock of 1 hour at 42 degrees Celsius resulted in mushroom development and an asymmetrical colony. In this study we performed a genome-wide expression analysis on dikaryons of wild-type (not heat shocked), delta-wc2 (heat shocked or not heat shocked) and the complemented strain delta-wc2 hsp3-wc2 (heat shocked or not heat shocked). A total of five samples were analyzed. One wild-type (not heat shocked), delta-wc2 (heat shocked or not heat shocked) and the complemented strain delta-wc2 hsp3-wc2 (heat shocked or not heat shocked).
Project description:RNA-seq profile of transgenic hindbrains expressing heat-shock induced constitutively activated fgfR1 (Tg(hsp70:ca-fgfr1)) and compared to heat-shocked counterparts Method: 22hpf embryos were heat-shocked for 30min at 38.5°C and then incubated for 2hr at 28.5°C . After hindbrains were microdissected and the RNA was extracted. fgfR1 overexpression was checked by qPCR and samples were selected for sequencing