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Measuring the dependence of the yeast heat shock response on intracellular pH and translation during stress

ABSTRACT: Measuring the dependence of the yeast heat shock response on intracellular pH and translation during stress

PROVIDER: PRJNA640923 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12062600_1.fastq.gz	Fastqsanger.gz
	SRR12062600_2.fastq.gz	Fastqsanger.gz
	SRR12062601_1.fastq.gz	Fastqsanger.gz
	SRR12062601_2.fastq.gz	Fastqsanger.gz
	SRR12062602_1.fastq.gz	Fastqsanger.gz

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Measuring the dependence of the yeast heat shock response on intracellular pH during stress

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Measuring the dependence of the yeast heat shock response on intracellular pH during stress

Project description:S. cerevisiae cells acidify when they experience stressful temperatures. To determine whether this heat-shock associated acidification is important for the cellular response, we manipulated intracellular pH, heat shocked cells, and sequenced the transcriptome.

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Measuring the dependence of the yeast heat shock response on intracellular pH and translation during stress

Project description:S. cerevisiae cells acidify when they experience stressful temperatures. In addition, newly-translated proteins are thought to misfold, triggering the heat shock response. To determine whether heat-shock associated acidification and translation state are important for the cellular response, we manipulated intracellular pH, blocked translation, heat shocked cells, and sequenced the transcriptome.

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