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POKI-Seq in Primary Human T Cells

ABSTRACT: We adapted pooled non-viral targeted knock-ins to allow for simultaneous readouts of pooled knock-in barcode as well as RNA transcriptomes at the single cell level in primary human T cells. We used a library of 36 potentially therapeutic knock-in constructs each knocked into the TRAC locus along with a new TCR specificity to explore transcriptional changes in therapeutically relvant in vitro and in vivo environments.

ORGANISM(S): Homo sapiens

PROVIDER: GSE143417 | GEO | 2020/05/18

REPOSITORIES: GEO

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