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Effects of PMN-Ectosomes on human macrophages

ABSTRACT: In order to define the inhibitory activity of PMN-Ectosomes, we investigated the early gene expression profiles of resting and zymosan A-stimulated human monocyte-derived macrophages in the absence or presence of PMN-Ectosomes.

ORGANISM(S): Homo sapiens

PROVIDER: GSE14419 | GEO | 2009/01/16

SECONDARY ACCESSION(S): PRJNA111503

REPOSITORIES: GEO

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Transcription profiling of human resting and zymosan A-stimulated monocyte-derived macrophages in the absence or presence of PMN-Ectosomes

Project description:In order to define the inhibitory activity of PMN-Ectosomes, we investigated the early gene expression profiles of resting and zymosan A-stimulated human monocyte-derived macrophages in the absence or presence of PMN-Ectosomes. Experiment Overall Design: We cultured monocytes isolated from fresh buffy coats of 4 healthy donors (2 males and 2 females between the ages of 20 to 45) for 7 days at 37Â°C. At day 7, macrophages were cultured for another 3 hours alone, in presence of PMN-Ectosomes, with zymosan A, and with zymosan A and PMN-Ectosomes.

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