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Calcium signaling as a possible mechanism behind increased locomotor response in zebrafish larvae exposed to a human relevant persistent organic pollutant mixture or PFOS

ABSTRACT: Persistent organic pollutants (POPs) are widespread in the environment and bioaccumulate in organisms. Previously we observed hyperactivity of zebrafish larvae exposed to a mixture of POPs based on average blood levels from the Scandinavian population and identified perfluorooctanesulfonic acid (PFOS) as the driving agent for the behavioral changes. We exposed zebrafish larvae from 6 to 96 h post fertilization to the same mixture of POPs in two concentrations or a single PFOS exposure (0.3 and 2.06mg/L) and performed behavioral tests and transcriptomics analysis. Results confirmed previous observations that exposure to POPs and PFOS causes hyperactivity and higher anxiety levels. Transcriptomic analysis showed upregulation of transcripts related to muscle contraction. Muscle contraction is highly regulated by the availability of calcium in the sarcoplasmic reticulum. Ingenuity pathway analysis showed that one of the affected pathways in larvae exposed to the POPs mixture and PFOS was calcium signalling via the activation of the ryanodine receptors. We also found effect on lipid metabolism in those larvae exposed to the lower concentration of PFOS. By using omics technology, we observed that the altered behavioral pattern in exposed zebrafish larvae might be controlled directly by mechanisms affecting muscle function rather than from mechanisms connected to neurotoxicity.

ORGANISM(S): Danio rerio

PROVIDER: GSE144525 | GEO | 2020/05/25

REPOSITORIES: GEO

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