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SINGLE FLOWER TRUSS and SELF-PRUNING differentially regulate developmental and metabolic genetic networks to guide cotton (Gossypium hirsutum) architectures [meristems]

ABSTRACT: Each plant's architecture, composed of patterns of indeterminate and determinate growth, is defined through the activities of meristems. Understanding the regulation of meristem identity can benefit plant architecture and crop yield. To understand how meristem activities contribute to different architectures in cotton (Gossypium hirsutum), we used RNA-Seq to determine the transcriptomes from meristems isolated from different developmental stages of wild photoperiodic and domesticated day-neutral cotton grown under different photoperiods.

ORGANISM(S): Gossypium hirsutum

PROVIDER: GSE144544 | GEO | 2020/06/30

REPOSITORIES: GEO

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SINGLE FLOWER TRUSS and SELF-PRUNING differentially regulate developmental and metabolic genetic networks to guide cotton (Gossypium hirsutum) architectures [GEO_SFT_SP]

Project description:Patterns of indeterminate and determinate growth specify plant architecture and influence crop productivity. In cotton (Gossypium hirsutum), SINGLE FLOWER TRUSS (SFT) stimulates the transition to flowering and determinate growth while its closely related antagonist SELF-PRUNING (SP) maintains meristems in indeterminate states to favor vegetative growth. To identify the molecular programs regulating cotton growth habits, and understand how altered expression of GhSFT and GhSP perturbs these programs, we used RNA-Seq to determine the gene expression networks involved in wild photoperiodic, domesticated day-neutral, and GhSFT- and GhSP-regulated architectures. GhSFT or GhSP affected the expression of genes regulating meristem fate and metabolic pathways. Gene ontology analysis predicted enrichment of transcripts involved in photosynthesis, secondary metabolism, and cell wall biosynthesis, and these hypotheses were functionally verified. Weighted gene co-expression network analyses using multiple cotton datasets identified diverse genetic modules highly correlated with SFT or SP, including those that may benefit crop management.

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SINGLE FLOWER TRUSS and SELF-PRUNING differentially regulate developmental and metabolic genetic networks to guide cotton (Gossypium hirsutum) architectures [meristems]

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