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Invasive Escherichia coli contributes to colon carcinogenesis by suppressing epithelial autophagy: application of phage therapy

ABSTRACT: Microbiota dysbiosis and mucosa-associated bacteria are involved in colorectal cancer (CRC) progression. However, the temporal changes in commensal-derived pathobionts and host-microbe interactions remain poorly understood. Our study demonstrated that antibiotic (ABX) treatment at a mid phase but not at early or late time points reduced tumor burden in a chemically induced CRC mouse model. A transient surge in fecal microbial richness and emergence of virulence factors were observed at the mid phase of CRC induction. Mouse colonic mucosal tissues were collected before (day 0) and on various days after administration of AOM/DSS (i.e., day 46 as early phase, day 56 as mid phase, and day 66 as late phase), and were analyzed using microarray. Unique profiles of autophagy-related genes were identified in the colonic mucosa during the mid phase, which correlated with the presence of intraepithelial bacteria.

ORGANISM(S): Mus musculus

PROVIDER: GSE144719 | GEO | 2021/11/24

REPOSITORIES: GEO

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