Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcriptomic profiling of wild type, sigG1 and rsfG mutants of Streptomyces tsukubaensis in liquid culture

ABSTRACT: The aim of this work was to compare gene expression of wild type, sigG1 and rsfG mutants, to determine the SigG1-dependent genes in Streptomyces

ORGANISM(S): Streptomyces tsukubensis NRRL18488

PROVIDER: GSE144815 | GEO | 2020/12/21

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Gene expression of wild type and lsr2 mutants of Streptomyces venezuelae during late development in liquid culture

Project description:Comparing gene expression of wild type and lsr2 mutants, to determine the effect of Lsr2 on Streptomyces development and metabolism

2018-06-09 | GSE115537 | GEO

Transcriptomic profiling of wild type, sigG1 and rsfG mutants of Streptomyces tsukubaensis in liquid culture

Project description:Transcriptomic profiling of wild type, sigG1 and rsfG mutants of Streptomyces tsukubaensis in liquid culture

| PRJNA604996 | ENA

Streptomyces tsukubaensis: transcriptomic response to tacrolimus repressing carbon sources

Project description:In this work, we identified glucose and glycerol as tacrolimus repressing carbon sources in the important species Streptomyces tsukubaensis. A genome-wide analysis of the transcriptomic response to glucose and glycerol additions was performed using microarray technology. The transcriptional time series obtained allowed us to compare the transcriptomic profiling of S. tsukubaensis growing under tacrolimus producing and non-producing conditions. The analysis revealed important and different metabolic changes after the additions and a lack of transcriptional activation of the fkb cluster. In addition, we detected important differences in the transcriptional response to glucose between S. tsukubaensis and the model species Streptomyces coelicolor. A number of genes encoding key players of morphological and biochemical differentiation were strongly and permanently downregulated by the carbon sources. Finally, we identified several genes showing transcriptional profiles highly correlated to that of the tacrolimus biosynthetic pathway regulator FkbN that might be potential candidates for the improvement of tacrolimus production

2017-10-12 | GSE99752 | GEO

Genome-wide transcriptome response of Streptomyces tsukubaensis to N-acetylglucosamine

Project description:Chitin is the second most abundant biopolymer present in soils and is utilized by antibiotic-producing Streptomyces species. Its monomer, N-acetylglucosamine (NAG), regulates the developmental program of the model organism Streptomyces coelicolor. NAG blocks differentiation when growing on rich medium whilst it promotes development on poor culture media. We report here the negative effect of NAG on tacrolimus (FK506) production in Streptomyces tsukubaensis NRRL 18488 growing on a defined rich medium. Using microarrays technology, we found that GlcNAc represses the transcription of fkbN, encoding the main transcriptional activator of the tacrolimus biosynthetic cluster, and of ppt1, encoding a phosphopantheteinyltransferase involved in tacrolimus biosynthesis. On the contrary, NAG stimulated transcription of genes related to amino acid and nucleotide biosynthesis, DNA replication, RNA translation, glycolysis, pyruvate metabolism, and key gene members of the PHO regulon. The results obtained support those previously reported for S. coelicolor, but some important differences were observed

2020-02-08 | GSE110393 | GEO

Identifying SigG1 binding sites in Streptomyces tsukubaensis

Project description:The aim of this work was to determine where SigG1 binds within the S. tsukubaensis chromosome

2020-12-21 | GSE144907 | GEO

Gene expression of wild type and lsr2 mutants of Streptomyces venezuelae during late development in liquid culture

Project description:Gene expression of wild type and lsr2 mutants of Streptomyces venezuelae during late development in liquid culture

| PRJNA475289 | ENA

Transcriptomic description of a classic model of carbon catabolic repression in Streptomyces coelicolor:Glk effect

Project description:The aim of this work was to use a high density microarray approach in Streptomyces coelicolor M145 and a glk substituted mutant (ScoZm)derived from it, to evaluate the paradigmatic model proposed for CCR in Streptomyces

2016-04-02 | E-MTAB-3602 | biostudies-arrayexpress

Streptomyces clavuligerus Transcriptome using adpA deleted and overexpressed mutants

Project description:RNA seq analysis was performed using adpA-deleted and adpA-overexpressed Streptomyces clavuligerus strains to determine its regulatory effect on tunicamycin and other secondary metabolites production

2024-07-01 | GSE243267 | GEO

Arabidopsis seedling hydroponic treatment with Streptomyces AGN23 culture supernatants

Project description:The objective of this study was to evaluate the effect of extracellular culture medium of Streptomyces AGN23, a strain inducing disease resistance in plants, on the Arabidopsis transcriptome.

2018-09-15 | GSE119986 | GEO

Expression analysis of Streptomyces avermitilis NRRL8165 delta-aveI mutant

Project description:Investigation of whole genome gene expression level changes in Streptomyces avermitilis delta-aveI mutant, compared to the wild-type strain. The mutants analyzed in this study are further described in Chen L, Lu Y., Chen J, Zhang W, Shu D, Qin Z, Yang S, Jiang W. (2008) Characterization of a negative regulator AveI for avermectin biosynthesis in Streptomyces avermitilis NRRL8165. Appl Microbiol Biotechnol 80(2): 277-86.

2009-07-02 | GSE16892 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data