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Transcriptomic description of a classic model of carbon catabolic repression in Streptomyces coelicolor:Glk effect

ABSTRACT: The aim of this work was to use a high density microarray approach in Streptomyces coelicolor M145 and a glk substituted mutant (ScoZm)derived from it, to evaluate the paradigmatic model proposed for CCR in Streptomyces

ORGANISM(S): S. coelicolor_M145_strain

SUBMITTER: alba romero

PROVIDER: E-MTAB-3602 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Transcriptomic analysis of a classical model of carbon catabolite regulation in Streptomyces coelicolor.

Romero-Rodríguez Alba A Rocha Diana D Ruiz-Villafan Beatriz B Tierrafría Víctor V Rodríguez-Sanoja Romina R Segura-González Daniel D Sánchez Sergio S

BMC microbiology 20160427

<h4>Background</h4>In the genus Streptomyces, one of the most remarkable control mechanisms of physiological processes is carbon catabolite repression (CCR). This mechanism regulates the expression of genes involved in the uptake and utilization of alternative carbon sources. CCR also affects the synthesis of secondary metabolites and morphological differentiation. Even when the outcome effect of CCR in different bacteria is the same, their essential mechanisms can be quite different. In several ...[more]

PMID: 27121083

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Transcriptomic description of a classic model of carbon catabolic repression in Streptomyces coelicolor: Glucose effect

Project description:The aim of this work was to use a high density microarray approach in Streptomyces coelicolor M145 using a represive condition (RM) (0.5%agar+0.5%glucose) and non-repressive condition (NRM) (agar 1%)

2016-04-02 | E-MTAB-3603 | biostudies-arrayexpress

Transcriptome data for Streptomyces coelicolor at 2 growth stage

Project description:Transcriptome analysis of Streptomyces coelicolor MT1110 cultures grown in SMM + 0.5% casaminoacids at 30?C up to mid exponential and stationary phase. Total RNA was extracted from both stages of growth, labelled with Cy3-dCTP and mixed with genomic DNA from S. coelicolor M145 labelled with Cy5-dCTP that was used as common reference in an indirect type of microarray experiment. Labelled Cy3/Cy5-dCTP samples were hybridized onto SCO-Chip2-v2 High Density IJISS arrays.

2020-08-01 | E-MAXD-61 | biostudies-arrayexpress

S.coelicolor_Rok7B7vsM145_MM+mannitol_time_course_expression

Project description:For transcript analysis, total RNA was isolated as described (Noens et al, 2007) from surface-grown mycelia of S. coelicolor M145 and its rok7B7 null mutant, cultivated on MM agar plates with mannitol (1% w/v) as the sole carbon source (plates were covered with cellophane discs). Two independent biological replicate experiments were performed. Samples were taken at 14h (early vegetative growth), 24 h (vegetative growth), 30 h (early aerial growth), 36 h (aerial growth), 42 h (early sporulation) and 54 h (sporulation). Total RNA was purified using the Kirby-mix protocol (Kieser et al, 2000). DNaseI treatment was used to fully remove any traces of DNA. Before use, the RNA samples were checked for integrity on an Agilent 2100 Bioanalyzer (Agilent Technologies). cDNA preparation and labeling was performed as previously described (Bucca et al, 1997, Bucca et al, 2009). The filtered data sets were analyzed using rank products analysis (Breitling et al, 2004) via the Rank-ProdIt tool (Laing and Smith, 2010). Differentially expressed genes were identified as demonstrating a > 2 fold change in expression between the parental strain M145 (wild-type) and the rok7B7 mutant.

2014-01-14 | E-MTAB-1465 | biostudies-arrayexpress

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Project description:The objective was to analyze the differential expression between the wild strain and the Streptomyces clavuligerus ΔclaR::aac mutant Six experimental conditions were assayed, two strains (Streptomyces clavuligerus ATCC 27064, S. clavuligerus ΔclaR::aac) in three culture times (22.5h, 46.5h and 60 h). Two biological replicates for each condition.

2015-08-05 | E-GEOD-66683 | biostudies-arrayexpress

Combined transcriptome analysis and polysome profiling of Streptomyces coelicolor MT1110 in SMMS

Project description:Total RNA, low ribosome density (LRD) and polysome associated RNA were obtained from parallel S.coelicolor cultures of wild type strain MT1110 grown on solid medium SMMS up to rapid growth phase II. The abundance of each RNA in the total RNA, the LRD or polysome pool was quantified by Cydye labeling and hybridization on high density whole genome 105K S.coelicolor DNA microarrays using S.coelicolor M145 as reference.

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2020-08-01 | E-MAXD-60 | biostudies-arrayexpress

sRNA scr5239 involved in important feedback loop of S. coelicolor central metabolism

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2020-01-28 | PXD016811 | Pride

Streptomyces coelicolor cells during various developmental stages: wildtype M145 vs. ÎSCO0204.

Project description:Global transcriptional profiling of the SCO0204 null mutant of Streptomyces coelicolor M145 in comparison to the wildtype M145. Goal was to determine the role of SCO0204.

2016-04-14 | E-MTAB-4597 | biostudies-arrayexpress

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2019-01-14 | E-MTAB-7457 | biostudies-arrayexpress

S. coelicolor M752 SMM medium time course study

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