Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Dnmt1 deletion in PV-positive cortical interneurons of 6 month and 18 month old mice [meDIP-seq]

ABSTRACT: The emerging role of epigenetic mechanisms like DNA methylation executed by DNA methyltransferases (DNMTs) in synaptic function irrevocably raises the question for the targeted subcellular processes and mechanisms. We here sequenced FAC-sorted PVergic interneurons of 6 month and 18month old PV-Cre/tdTomato/Dnmt1loxp wildtype and knockout mice and performed MeDIP-Seq.

ORGANISM(S): Mus musculus

PROVIDER: GSE145024 | GEO | 2020/07/06

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Dnmt1 deletion in PV-positive cortical interneurons of 6 month and 18 month old mice

Project description:The emerging role of epigenetic mechanisms like DNA methylation executed by DNA methyltransferases (DNMTs) in synaptic function irrevocably raises the question for the targeted subcellular processes and mechanisms. We here sequenced FAC-sorted PVergic interneurons of 6 month and 18month old PV-Cre/tdTomato/Dnmt1loxp wildtype and knockout mice and performed RNA-Seq and MeDIP-Seq.

2020-07-06 | GSE145026 | GEO

Dnmt1 deletion in PV-positive cortical interneurons of 6 month and 18 month old mice [RNA-seq]

2020-07-06 | GSE145025 | GEO

Dnmt1 deletion in Hmx3-positive POA-derived interneurons [MeDIP-seq]

Project description:The proliferative niches in the subpallium generate a rich cellular variety fated for diverse telencephalic regions. The embryonic preoptic area (POA) represents one of these domains giving rise to the pool of cortical GABAergic interneurons and glial cells, in addition to striatal and residual POA cells. The migration from sites of origin within the subpallium to the distant targets like the cerebral cortex, accomplished by the adoption and maintenance of a particular migratory morphology, is a critical step during interneuron development, which seems to be regulated partially via DNA methylation-dependent gene expression. To identify genes that are altered by DNA methylation mediated by DNMT1 in POA-derived Hmx3-positive interneurons, we used an Hmx3-Cre/tdTomato/Dnmt1loxP mouse model and FAC-sorted the basal telencephalon at E16. RNA and MeDIP sequencing were performed. MeDIP data are assigned here.

2021-03-09 | GSE146967 | GEO

Dnmt1 deletion in Hmx3-positive POA-derived interneurons [RNA-seq]

2021-03-09 | GSE146966 | GEO

Dnmt1 deletion in PV-positive cortical interneurons of 6 month and 18 month old mice [meDIP-seq]

Project description:Dnmt1 deletion in PV-positive cortical interneurons of 6 month and 18 month old mice [meDIP-seq]

| PRJNA605744 | ENA

Dnmt1 deletion in PV-positive cortical interneurons of 6 month and 18 month old mice

Project description:Dnmt1 deletion in PV-positive cortical interneurons of 6 month and 18 month old mice

| PRJNA605736 | ENA

Dnmt1 deletion in PV-positive cortical interneurons of 6 month and 18 month old mice [RNA-seq]

Project description:Dnmt1 deletion in PV-positive cortical interneurons of 6 month and 18 month old mice [RNA-seq]

| PRJNA605743 | ENA

Dnmt1 deletion in Hmx3-positive POA-derived interneurons [MeDIP-seq]

Project description:Dnmt1 deletion in Hmx3-positive POA-derived interneurons [MeDIP-seq]

| PRJNA612524 | ENA

MEF2C CUT&RUN in PV and SST interneurons.

Project description:CUT&RUN for MEF2C in mature PV+ and SST+ cortical interneurons to characterize the differential usage of this transcription factor by these populations.

2021-09-22 | GSE165018 | GEO

Dnmt1 has de novo activity targeted to transposable elements

Project description:DNA methylation plays a critical role in development, particularly in repressing retrotransposons. The mammalian methylation landscape is dependent on the combined activities of the canonical maintenance enzyme Dnmt1 and the de novo Dnmts, 3a and 3b. Here we demonstrate that Dnmt1 displays de novo methylation activity in vitro and in vivo with specific retrotransposon targeting. We used whole-genome bisulfite and long-read Nanopore sequencing in genetically engineered methylation depleted embryonic stem cells to provide an in-depth assessment and quantification of this activity. Utilizing additional knockout lines and molecular characterization, we show that Dnmt1's de novo methylation activity depends on Uhrf1 and its genomic recruitment overlaps with targets that enrich for Trim28 and H3K9 trimethylation. Our data demonstrate that Dnmt1 can de novo add and maintain DNA methylation, especially at retrotransposons and that this mechanism may provide additional stability for long-term repression and epigenetic propagation throughout development.

2021-06-29 | PXD025736 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data