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Genomic location of H4R3me2a and H3R2me2s in chicken polychromatic erythrocytes

ABSTRACT: H4R3me2a and H3R2me2s often track together, locate to transcriptionally active DNA and are at regulatory regions of expressed genes.

ORGANISM(S): Gallus gallus

PROVIDER: GSE145398 | GEO | 2020/03/09

REPOSITORIES: GEO

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Genomic location of H4R3me2a and H3R2me2s in chicken polychromatic erythrocytes

Project description:Genomic location of H4R3me2a and H3R2me2s in chicken polychromatic erythrocytes

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Mapping of low-salt soluble chromatin identifies known and novel transcriptionally active genes in chicken erythrocytes

Project description:Transcriptional regulation is regulated by the organization of the genome into chromatin compartments and domains. Here, we present the detailed characteristics, including location of nucleosome-free regions, of several novel transcriptionally active/poised chromatin compartments exhibiting a range of transcriptional activities. These chromatin patterns illustrate how the salt solubility profile of a genomic region aids in the annotation of genes expressed in erythroid cells and contributes to the identification of functional features such as regulatory regions.

2019-07-24 | GSE117595 | GEO

Mapping of low-salt soluble chromatin identifies known and novel transcriptionally active genes in chicken erythrocytes

Project description:Mapping of low-salt soluble chromatin identifies known and novel transcriptionally active genes in chicken erythrocytes

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Atypical chromatin structure of immune-related genes expressed in chicken erythrocytes [RNA-seq]

Project description:Transcriptional regulation is impacted by multiple layers of genome organization. Here, we report that immune genes were associated with H3K4me3 and H3K27ac. In conjunction with next-generation DNA, RNA sequencing, chromatin immunoprecipitation-DNA sequencing and FAIRE sequencing, we identified the active chromosomal compartments and determined their structural signatures in relation to expression levels.

2020-12-31 | GSE141014 | GEO

The chicken immature erythrocyte transcriptome

Project description:Transcriptional regulation is impacted by multiple layers of genome organization. Here, we identified and mapped out all the transcriptionally active chromosomal domains in the chicken immature erythrocyte genome, including the known β- and α-globin domains, by combining a powerful chromatin fractionation method with next generation DNA and RNA sequencing.

2016-01-01 | GSE75848 | GEO

The chicken immature erythrocyte transcriptome

Project description:Transcriptional regulation is impacted by multiple layers of genome organization. Here, we identified and mapped out all the transcriptionally active chromosomal domains in the chicken immature erythrocyte genome, including the known Î²- and Î±-globin domains, by combining a powerful chromatin fractionation method with next generation DNA and RNA sequencing. Two biological replicates of total RNA were sequenced to characterize genes in chicken erythrocyte cells

2016-07-03 | E-GEOD-75848 | biostudies-arrayexpress

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Transcripts of antibacterial peptides in chicken erythrocytes infected with Marek's disease virus.

Project description:BACKGROUND:Chicken erythrocytes are involved in immunity through binding of toll-like receptors (TLRs) with their ligands to activate downstream signaling and lead to cytokine production in erythrocytes. Some avian ?-defensins (AvBDs) are constitutively expressed in tissues and some others can be induced by various bacteria and viruses. However, the expression of AvBDs in erythrocytes has not yet been studied extensively. RESULTS:The transcripts of eight AvBDs (AvBD1 to AvBD7, and AvBD9) and liver-expressed antimicrobial peptide-2 (LEAP-2) were found in normal chicken erythrocytes. The expression levels of AvBD2, 4 and 7 were significantly increased (P?<?0.01), whereas the levels of AvBD1, 6 and 9 were significantly decreased (P?<?0.01) after Marek's disease virus (MDV) infection. The mRNA expression level of LEAP-2 was not significantly changed after MDV infection. Highest viral nucleic acid (VNA) of MDV in the feather tips among the tested time points was found at 14 days post-infection (d.p.i.). In addition, 35 MD5-related gene segments were detected in the erythrocytes at 14 d.p.i. by transcriptome sequencing. CONCLUSIONS:These results suggest that the AvBDs in chicken erythrocytes may participate in MDV-induced host immune responses.

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Chicken erythrocytes stimulated with LPS, PGN and Poly(I:C)

Project description:Erythrocytes represent the most abundant cell type of the bloodstream in all vertebrates. The principal feature that differenciates erythrocytes of non-mammals is that unlike mammals they mantain the nucleus in their terminal differentiation. While the main function associated to erythrocytes is the oxygen and carbon dioxide transport, some other functions, no less important, have been attributed to these cells. The focus of this study was to investigate the response of cultured chicken erythrocytes to bacterial lipopolysaccharide (LPS), bacterial peptidoglycan (PGN) and to the analog viral dsRNA Poly(I:C) using a comercial microarray platform from Agilent.

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Proviral location affects cognate peptide-induced virus production and immune recognition of 2 HIV-1-infected T cell clones

Project description:In this study, we determined the effect of proviral inducibility on CD8+ T cell recognition of HIV-infected clones. We showed that a T cell clone containing a provirus integrated in relatively transcriptionally inactive site was much less recognized by CD8+ T cells than a T cell clone containing a provirus that was integrated into a trancriptionally active site.

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