Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Genome-wide analysis of normal and high glucose condition

ABSTRACT: Transcriptional profiling of Mouse cavernous primary pericytes (MCPs) in comparing normal glucose condition with high glucose condition

ORGANISM(S): Mus musculus

PROVIDER: GSE146357 | GEO | 2021/01/21

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Transcriptional profiling of mouse cavenous endothelial cells in normal and high glucose condition

Project description:Purpose: To investigate the potential target genes associated with diabetic condition in mouse cavernous endothelial cells (MCECs) for diabetic induced erectile dysfunction (ED) treatment. Materials and Methods: Mouse cavernous tissue was embedded into Matrigel and sprouted cells were subcultivated for other studies. To mimic diabetic condition, the MCECs were exposed to normal-glucose (NG, 5 mmoL) and high-glucose (HG, 30 mmoL) condition for 72 hours. RNA-sequencing assay was performed to evaluate gene expression profiling and RT-PCR was used for sequencing data validation.

2020-09-01 | GSE146078 | GEO

Genome-wide analysis of High glucose-induced gene expression by Ninjurin1 siRNA

2014-05-20 | GSE57525 | GEO

Genome-wide analysis of High glucose-induced gene expression by DKK2 protein

2018-05-04 | GSE95178 | GEO

Genome-wide analysis of High glucose-induced gene expression by Ninjurin1 siRNA

Project description:Transcriptional profiling of Mouse cavernous primary endothelial cells (MCECs) in comparing normal glucose condition with high glucose condition or Ninjurin1 siRNA transfection condition. Objective was to determine the effect of ninjurin1 on high glucose conditioned cells on global gene expression. Total RNA obtained from MCECs, which were treated with High glucose (HG) condition or (HG+ninjurin1 siRNA). Biological replicates: 1 control replicates, 1 HG replicates, 1 (HG+ninjurin1 siRNA) replicates.

2014-05-20 | E-GEOD-57525 | biostudies-arrayexpress

Genome-wide analysis of normal and high glucose condition

Project description:Genome-wide analysis of normal and high glucose condition

| PRJNA610247 | ENA

MicroRNA-148a-3p in pericyte-derived extracellular vesicles improves erectile function in diabetic mice by promoting cavernous neurovascular regeneration

Project description:Purpose: To investigate the regulatory role of microRNA (miR)-148a-3p in mouse corpus cavernous pericyte (MCPs)-derived extracellular vesicles (EVs) in the treatment of diabetes-induced erectile dysfunction (ED). Materials and Methods: Mouse corpus cavernous tissue was used for MCPs primary culture and EVs isolation. Small RNA sequencing analysis was performed to assess the type and content of miRs in MCPs-EVs. Four groups of mice were used: control nondiabetic mice and streptozotocin-induced diabetic mice receiving two intracavernous injections (days -3 and 0) of phosphate buffered saline, MCPs-EVs transfected with regent control, or MCPs-EVs transfected with miR-148a-3p inhibitor. The function of miR-148a-3p in MCPs-EVs was evaluated by tube formation assay, migration assay, TUNEL assay, intracavernous pressure, immunofluorescence staining, and western blot experiments. Results: We extracted EVs from MCPs and by small RNA sequencing analysis we found that miR-148a-3p is enriched in MCPs-EVs. Exogenous administration of MCPs-EVs can effectively promote mouse cavernous endothelial cell (MCECs) tube formation, migration, proliferation, and reduce MCECs apoptosis under high-glucose conditions. However, these effects are significantly attenuated in miR-148a-3p-depleted MCPs-EVs, which is extracted after inhibiting miR-148a-3p expression in MCPs. Repeated intracavernous injections of MCPs-EVs improves erectile function by inducing cavernous neurovascular regeneration in diabetic mice. Through online bioinformatics databases and luciferase report assays, we predict Pyruvate dehydrogenase kinase-4 (PDK4) is a potential target gene of miR-148a-3p. Conclusions: Our findings provide new and reliable evidence that miR-148a-3p in MCPs-EVs significantly enhances cavernous neurovascular regeneration by inhibiting PDK4 in diabetic mice.

2023-12-19 | GSE195533 | GEO

Transcriptional profiling of mouse cavenous endothelial cells in normal and high glucose condition

Project description:Transcriptional profiling of mouse cavenous endothelial cells in normal and high glucose condition

| PRJNA609141 | ENA

Expression data from TCF19 knockdown in HepG2 cells maintained under high glucose (40mM) condition

Project description:Changes in concentration of glucose in the cellular environment results in a variety of changes in the transcription program. Liver is the primary organ for metabolic regulation in the body, and hence, any surge in circulating blood glucose leads to changes in transcriptional states of important enzymes tasked to maintain metabolic homeostasis. Chromatin modification reader proteins play an important role in maintaining metabolic homeostasis by identification of the histone modification, and facilitating recruitment of chromatin remodelling enzymes. We knocked down an important reader domain containing protein, TCF19, in human hepatocellular carcinoma cells ( HepG2) maintained under high glucose condition and used microarray to investigate the changes in gene expression profile.

2018-02-13 | GSE107471 | GEO

CutnRun of O-GlcNac in mouse ES cells in high glucose condition

Project description:CutnRun of O-GlcNac in mouse ES cells in high glucose condition

| PRJEB78514 | ENA

HK-2 cell cultured with normal glucose and high glucose

Project description:HK-2 cell cultured with normal glucose and high glucose

| PRJNA707218 | ENA