Plant cell wall and development. Comparison of transcript levels between elongating 5 and 11 day-old plants
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ABSTRACT:
ORGANISM(S): Arabidopsis thaliana
PROVIDER: GSE14648 | GEO | 2009/03/01
SECONDARY ACCESSION(S): PRJNA112309
REPOSITORIES: GEO
Similar Datasets
Project description:rs05-11_hypocotyls - hypocotyls - Fine regulation of genes specifically expressed during cell elongation. - Seeds of A. thaliana were sowed on culture medium poured in Magenta boxes. They were allowed to germinate. Plantlets were in vitro for 5 or 11 days and harvested for RNA extraction. Keywords: organ comparison 3 dye-swap - CATMA arrays
2009-03-01 | E-GEOD-14648 | biostudies-arrayexpress
Project description:Seeds of A. thaliana were sowed on culture medium poured in Magenta boxes. They were allowed to germinate. Plantlets were in vitro for 5 or 11 days and harvested for RNA extraction.
2007-07-17 | E-MEXP-789 | biostudies-arrayexpress
Project description:rs05-12_setii - all comparison - Differential transcriptome in mutant plants - seeds were sown in vitro and plantlets (Col0 wild type ,and n1, n2, n3, x123, sdg8, vip4,sdg25 and sdg26 mutants)were cultivated during 6 days (1.02 boyes stadge) Keywords: gene knock out
2007-07-20 | GSE8427 | GEO
Project description:rs05-12_setii - sdg25 and sdg26 - Differential transcriptome in mutant plants - seeds were sown in vitro and plantlets (Col0 wild type ,and n1, n2, n3, x123, sdg8, vip4,sdg25 and sdg26 mutants)were cultivated during 6 days (1.02 boyes stadge) Keywords: gene knock out
2007-07-20 | GSE8429 | GEO
Project description:Investigation of the effect of atpme3-1 mutant on whole gene expression level hypocotyls of 96 hours-old plantlets were analyzed for this study
2016-02-29 | GSE75326 | GEO
Project description:rs05-05_atrazine - effect of atrazine and sucrose on arabidopsis transcriptome - Growth in the presence of sucrose was shown to confer to Arabidopsis thaliana seedlings, under conditions of in vitro culture, a very high level of tolerance to the herbicide atrazine and to other photosynthesis inhibitors (Sulmon et al., 2004). The CATMA investigation will be useful to reveal the gene networks implicated in the mechanisms of xenobiotics tolerance. - Seeds of Arabidopsis thaliana (ecotype Columbia) were surface-sterilized inbayrochlore/ethanol (1:1, v/v), rinsed in absolute ethanol and dried overnight. Germination and growth were carried out under axenic conditions in square Petri dishes. After seeds were sowed, Petri dishes were placed at 4degreeC for 48 h in order to break dormancy and homogenize germination, and then transferred to a control growth chamber at 22degreeC under a 16-h light period regime at 4500 lux for 4 weeks. Growth medium consisted of 0.8% (w/v) agar in 1x Murashige and Skoog (MS) basal salt mix (Sigma, St. Louis, MO, USA) adjusted to pH 5.7. After 4 weeks of cultivation on vertical plates plantlets were transferred on fresh medium complemented or not with atrazine 10 uM and with sucrose 80mM or mannitol 80 mM which were directly added during preparation of agar-MS media prior to sterilisation. Atrazine was sterilized by microfiltration through 0.2 um cellulose acetate filters (Polylabo, Strasbourg, France) and then axenically added to melted agar-MS medium prior to pouring into Petri dishes. Then plantlets were harvested after 24 hours of transfer and extracted for RNA. Keywords: treated vs untreated comparison
2007-05-31 | GSE7942 | GEO
Project description:Potato seedlings were subjected to cold, heat and salt stress. Expression profiles were captured at three different time-points, 3h, 9h and 27h from two different tissues, roots and leaves. The experiment was preformed independently three times. Commercially available true potato seeds (Variety Gilroy) were germinated on rafts floating on hydroponic medium in Magenta boxes. Plants were grown for 5 weeks prior to stress application under long day conditions (16h light and 8h dark) at 25C with gentle agitation. To initiate stress the medium was replaced with fresh medium pre-chilled to 4C (cold stress), pre-heated to 35C (heat stress) or supplemented with 100mM NaCl (salt stress). Cold and heat stress were maintained for the duration of the experiment by placing the Magenta boxes on ice or in a water-bath at 35C. For every individual sample two boxes of plants were used pooling a total of 6 plants per sample. For each time-point a single control sample was used by changing the media in a similar way as for the stress induction. A total of six boxes were combined for the pooled reference samples. Plants were harvested at the appropriate time and snap-frozen in liquid nitrogen. Roots and aerial tissue was separated prior to freezing. The tissue was stored at -80C freezer until isolation. Total RNA was isolated using RNeasy isolation kit. The intactness of the RNA was verified on gel and the concentration was adjusted to 3ug/ul by ethanol precipitation and re-suspension. Series_weblink: http://www.tigr.org/tdb/potato Keywords = potato, Abiotic stress
2010-06-10 | E-GEOD-2416 | biostudies-arrayexpress
Project description:Potato seedlings were subjected to cold, heat and salt stress. Expression profiles were captured at three different time-points, 3h, 9h and 27h from two different tissues, roots and leaves. The experiment was preformed independently three times. Commercially available true potato seeds (Variety Gilroy) were germinated on rafts floating on hydroponic medium in Magenta boxes. Plants were grown for 5 weeks prior to stress application under long day conditions (16h light and 8h dark) at 25C with gentle agitation. To initiate stress the medium was replaced with fresh medium pre-chilled to 4C (cold stress), pre-heated to 35C (heat stress) or supplemented with 100mM NaCl (salt stress). Cold and heat stress were maintained for the duration of the experiment by placing the Magenta boxes on ice or in a water-bath at 35C. For every individual sample two boxes of plants were used pooling a total of 6 plants per sample. For each time-point a single control sample was used by changing the media in a similar way as for the stress induction. A total of six boxes were combined for the pooled reference samples. Plants were harvested at the appropriate time and snap-frozen in liquid nitrogen. Roots and aerial tissue was separated prior to freezing. The tissue was stored at -80C freezer until isolation. Total RNA was isolated using RNeasy isolation kit. The intactness of the RNA was verified on gel and the concentration was adjusted to 3ug/ul by ethanol precipitation and re-suspension. Keywords: Direct comparison
2007-09-01 | GSE8205 | GEO
Project description:Potato seedlings were subjected to cold, heat and salt stress. Expression profiles were captured at three different time-points, 3h, 9h and 27h from two different tissues, roots and leaves. The experiment was preformed independently three times. Commercially available true potato seeds (Variety Gilroy) were germinated on rafts floating on hydroponic medium in Magenta boxes. Plants were grown for 5 weeks prior to stress application under long day conditions (16h light and 8h dark) at 25C with gentle agitation. To initiate stress the medium was replaced with fresh medium pre-chilled to 4C (cold stress), pre-heated to 35C (heat stress) or supplemented with 100mM NaCl (salt stress). Cold and heat stress were maintained for the duration of the experiment by placing the Magenta boxes on ice or in a water-bath at 35C. For every individual sample two boxes of plants were used pooling a total of 6 plants per sample. For each time-point a single control sample was used by changing the media in a similar way as for the stress induction. A total of six boxes were combined for the pooled reference samples. Plants were harvested at the appropriate time and snap-frozen in liquid nitrogen. Roots and aerial tissue was separated prior to freezing. The tissue was stored at -80C freezer until isolation. Total RNA was isolated using RNeasy isolation kit. The intactness of the RNA was verified on gel and the concentration was adjusted to 3ug/ul by ethanol precipitation and re-suspension. Series_weblink: http://www.tigr.org/tdb/potato Keywords = potato, Abiotic stress Keywords: ordered
2005-03-19 | GSE2416 | GEO
Project description:Investigation of the effect of atpme3-1 mutant on whole gene expression level hypocotyls of 96 hours-old plantlets were analyzed for this study A 12plex high density chip was used. Six or 5 biological replicates for each condition. Each replicate was obtained separately from the others and each replicate represents about 300 hypocotyles.
2016-02-29 | E-GEOD-75326 | biostudies-arrayexpress