Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Expression profile of miRNAs in Extracellular vesicles (EVs) obtained from Bovine granulosa cells subjected to heats stress

ABSTRACT: The study aimed to uncover the release of miRNAs via EVs and the differential release of these miRNAs from bovine granulosa cells in response to heat stress

ORGANISM(S): Bos taurus

PROVIDER: GSE146491 | GEO | 2020/09/30

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Expression profile of miRNAs in Extracellular vesicles (EVs) obtained from Bovine granulosa cells subjected to heats stress

Project description:Expression profile of miRNAs in Extracellular vesicles (EVs) obtained from Bovine granulosa cells subjected to heats stress

| PRJNA610729 | ENA

NanoString assays of miRNA in MCF7 and MCF10A cells and in extracellular vesicles (EVs) derived from these cells.

Project description:The goal of this study is to identify unique miRNA profiles of EVs from MCF7 and MCF10A cells that distinguish their cellular origin. 654 human mature miRNAs were analyzed in NanoString assays to identify miRNA with high abundance in MCF7 EVs and the greatest fold change for MCF7 EVs relative to MCF10A EVs.

2015-02-21 | E-GEOD-66165 | biostudies-arrayexpress

Proteome of bovine granulosa cells and extracellular vesicles from follicular fluid

Project description:The follicular fluid (FF) fills the interior of ovarian antral follicle and provides the microenvironment for oocyte growth and acquisition of its competence to ovulate and latter support early embryo development. The FF is derived from both blood plasma and secretion of different types of follicular cells. It contains also extracellular vesicles (EVs), including exosomes, small membrane-coated EVs with 30-150 nm in diameter, which participate in cell-to cell communication and signaling by transferring their cargo of different types of RNAs, proteins and lipids into the oocyte or follicular cells. To date most studies have focused on studying the ffEVs miRNAs cargo and showing that miRNAs can influence oocyte competence and further embryo development. However, ffEVs protein cargo, which could have a direct contribution after being uptake by the oocyte or follicular cells have been less studies.

2021-08-17 | PXD020508 | Pride

MicroRNA microarray between smoking-induced human bronchial epithelial cell (HBEC)-derived extracellular vesicles (EVs) and non-treated HBEC-derived Evs

Project description:To identify exosomal miRNAs that regulate smoking-induced lung myofibroblast differentiation, we conducted a miRNA microarray between smoking-induced HBEC-derived EVs and non-treated HBEC-derived EVs.

2018-02-19 | GSE66119 | GEO

Expression profile of miRNAs in Bovine granulosa cells subjected to heats stress

Project description:The study aimed to uncover differential expression pattern of microRNAs in bovine granulosa cells subjected to heat stress

2020-09-30 | GSE146490 | GEO

Gene expression in bovine ovarian follicle granulosa

2014-05-20 | GSE39589 | GEO

Gene expression in bovine ovarian follicle granulosa

Project description:Granulosa cells mature and die as ovarian follicles enlarge and die (undergo atresia) under the influence of hormones and intrafollicular factors. Later in follicular development, a fluid-filled antrum is formed, a process which is accompanied by a high rate of atresia. These small antral follicles (5 mm or less in diameter in the cow) contain granulosa of 2 different phenotypes, rounded or columnar, whereas follicles larger than 5 mm have the rounded phenotype only. Prior to ovulation, in larger follicles greater than 10 mm in size, the granulosa begin to migrate and differentiate in preparation for oocyte release and formation of the corpus luteum. These two key phases of follicular development were studied by gene expression microarray analysis using a bovine model to dissect the molecular mechanisms underlying these processes. Four groups of bovine ovarian follicles were selected for analysis. Follicle size, type and array number for each group are as follows: small (3-5 mm) healthy rounded (n=5), small healthy columnar (n=5), atretic (n=5) and large healthy (>10 mm; n=4). For each group, the RNA from a single follicle was used to hybridise an array, except for 3 small healthy samples which were pooled from 2 follicles each due to low RNA.

2014-05-20 | E-GEOD-39589 | biostudies-arrayexpress

Expression profile of miRNAs in Bovine granulosa cells subjected to heats stress

Project description:Expression profile of miRNAs in Bovine granulosa cells subjected to heats stress

| PRJNA610728 | ENA

Serum-free media supplements carry miRNAs that co-purify with extracellular vesicles (EVs)

Project description:With the goal to identify miRNAs and other small RNAs involved in EV-mediated oligodendrocyte-neuron communication, we performed deep sequencing (RNA-seq) of small RNAs derived from EVs isolated from culture supernatants of primary mouse oligodendrocytes by differential centrifugation. Oligodendrocytes were cultured and EVs harvested under serum-free conditions in presence of B27 supplement. We found that RNA isolated from EVs harvested from cells under serum-replacement conditions contains miRNA contaminants carried into the sample by defined media components.

2019-08-15 | GSE134096 | GEO

Differentially enriched miRNAs in EVs derived from young and aged osteocytes of mice

Project description:Vascular calcification often occurs with osteoporosis, a contradictory association called “calcification paradox”. We find that extracellular vesicles (EVs) released from aged bone matrix (AB-EVs) during bone resorption favor adipogenesis rather than osteogenesis of BMSCs and augment calcification of vascular smooth muscle cells (VSMCs). Intravenous or intramedullary injection of AB-EVs promotes bone-fat imbalance and exacerbates Vitamin D3 (VD3)-induced vascular calcification in young or old mice. To explore the involvement of miRNAs in the AB-EVs-induced promotion of adipocyte formation and vascular calcification, the Agilent miRNA array was conducted to compare the miRNA expression profiles in AB-EVs and YB-EVs from mouse bone specimens. Our study uncovers the role of AB-EVs as a messenger for calcification paradox by transferring functional miRNAs.

2022-02-15 | E-MTAB-11417 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data