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Expression profile of miRNAs in Extracellular vesicles (EVs) obtained from Bovine granulosa cells subjected to heats stress

ABSTRACT: Expression profile of miRNAs in Extracellular vesicles (EVs) obtained from Bovine granulosa cells subjected to heats stress

PROVIDER: PRJNA610729 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR11249229.fastq.gz	Fastqsanger.gz
	SRR11249230.fastq.gz	Fastqsanger.gz
	SRR11249231.fastq.gz	Fastqsanger.gz
	SRR11249232.fastq.gz	Fastqsanger.gz
	SRR11249233.fastq.gz	Fastqsanger.gz

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Expression profile of miRNAs in Extracellular vesicles (EVs) obtained from Bovine granulosa cells subjected to heats stress

Project description:The study aimed to uncover the release of miRNAs via EVs and the differential release of these miRNAs from bovine granulosa cells in response to heat stress

2020-09-30 | GSE146491 | GEO

Expression profile of miRNAs in Bovine granulosa cells subjected to heats stress

Project description:The study aimed to uncover differential expression pattern of microRNAs in bovine granulosa cells subjected to heat stress

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Expression profile of miRNAs in Bovine granulosa cells subjected to heats stress

Project description:Expression profile of miRNAs in Bovine granulosa cells subjected to heats stress

| PRJNA610728 | ENA

Serum-free media supplements carry miRNAs that co-purify with extracellular vesicles (EVs)

Project description:With the goal to identify miRNAs and other small RNAs involved in EV-mediated oligodendrocyte-neuron communication, we performed deep sequencing (RNA-seq) of small RNAs derived from EVs isolated from culture supernatants of primary mouse oligodendrocytes by differential centrifugation. Oligodendrocytes were cultured and EVs harvested under serum-free conditions in presence of B27 supplement. We found that RNA isolated from EVs harvested from cells under serum-replacement conditions contains miRNA contaminants carried into the sample by defined media components.

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Differentially enriched miRNAs in EVs derived from young and aged osteocytes of mice

Project description:Vascular calcification often occurs with osteoporosis, a contradictory association called “calcification paradox”. We find that extracellular vesicles (EVs) released from aged bone matrix (AB-EVs) during bone resorption favor adipogenesis rather than osteogenesis of BMSCs and augment calcification of vascular smooth muscle cells (VSMCs). Intravenous or intramedullary injection of AB-EVs promotes bone-fat imbalance and exacerbates Vitamin D3 (VD3)-induced vascular calcification in young or old mice. To explore the involvement of miRNAs in the AB-EVs-induced promotion of adipocyte formation and vascular calcification, the Agilent miRNA array was conducted to compare the miRNA expression profiles in AB-EVs and YB-EVs from mouse bone specimens. Our study uncovers the role of AB-EVs as a messenger for calcification paradox by transferring functional miRNAs.

2022-02-15 | E-MTAB-11417 | biostudies-arrayexpress

Crosstalk between the effects of extracellular vesicles (Evs) and TNF on U937 human monocyte cell line

Project description:Under physiological conditions, extracellular vesicles (EVs) are present simultaneously in the extracellular compartment together with cytokines. Thus, we hypothesized that EVs in combination with cytokines induce different responses of monocyte cells compared to EVs or cytokines alone. Human monocyte U937 cells were incubated with EV-containing or EV-free CCRF human T-cell supernatant, with or without the addition of TNF. U937 cells cultured in EV-free supernatant, supernatant containing CCRF t-cell derived EVs, TNF or both. Each treatment option was measured in 3 replicates.

2013-06-19 | E-GEOD-47897 | biostudies-arrayexpress

NanoString assays of miRNA in MCF7 and MCF10A cells and in extracellular vesicles (EVs) derived from these cells.

Project description:The goal of this study is to identify unique miRNA profiles of EVs from MCF7 and MCF10A cells that distinguish their cellular origin. 654 human mature miRNAs were analyzed in NanoString assays to identify miRNA with high abundance in MCF7 EVs and the greatest fold change for MCF7 EVs relative to MCF10A EVs.

2015-02-21 | E-GEOD-66165 | biostudies-arrayexpress

Identification miRNA expression profiles of human granulosa cells obtained from PCOS patients and control individuals

Project description:The miRNA expression profiles of mural granulosa cells (MGCs) which were obtained from PCOS patients and control individuals were identified and compared.

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Comparison of EVs isolation methods for miRNAs sequencing

Project description:Background & aims: MicroRNAs (miRNAs) encapsulated in EVs are potential diagnostic and prognostic biomarkers. However, discrepancies on miRNA patterns and their validation are still frequent due to differences in sample origin, EVs isolation, miRNA extraction and sequencing methods. Selecting appropriate EVs isolation methods is therefore a critical step for miRNA-based biomarker discovery. The aim of the present study is to find the most suitable EVs isolation method for miRNAs sequencing adequate for clinical application. Material & Methods EVs were isolated by Size Exclusion Chromatography (SEC), iodixanol gradients (GRAD) and the combination of both (SEC+GRAD), using the same plasma sample, in triplicate isolation assays. Isolated EVs were characterized and RNA was extracted. Three different protocols for miRNA library preparation were compared (NEBNext, NEXTFlex and SMARTer smRNA-seq) and miRNAs encapsulated on EVs were sequenced using NextSeq 500 system (Illumina). Finally, the yield, abundance and diversity of miRNAs using the three different EVs isolation protocols were analyzed and compared between them. Results The majority of lipoproteins, total cholesterol and plasma proteins were removed from the EVs-containing fractions by using SEC, GRAD, and SEC+GRAD. SEC method recovered a larger amount of EVs followed by GRAD and SEC+GRAD, while GRAD and SEC+GRAD yielded the purest vesicles. NEBNext was the library preparation kit that showed the highest reproducibility among replicas, higher number of reads corresponding to miRNAs and more different miRNAs, followed by NEXTFlex and SMARTer smRNA-seq. GRAD method showed the highest reproducibility among replicas, a higher number of reads corresponding to miRNAs and more different miRNAs, followed by SEC and SEC+GRAD methods. Conclusions These results render the GRAD method to isolate EVs as one of the most appropriate to detect miRNAs from Evs.

2023-04-26 | GSE223838 | GEO

Extracellular Vesicles (EVs) derived from Hemangioma stem cells (HemSC)

Project description:In order to investigate the function and mechanism of HemSC-EVs, we performed RNA-seq of HemSC-EVs which were extracted from cell culture supernatant of HemSC.

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