Transcriptomics

Dataset Information

0

RNase H2, mutated in Aicardi‐Goutières syndrome, resolves co-transcriptional R-loops to prevent DNA breaks and inflammation.


ABSTRACT: RNase H2 is a specialized enzyme that degrades RNA in RNA/DNA hybrids and deficiency of this enzyme causes a severe neuroinflammatory disease, Aicardi Goutières syndrome (AGS). However, the molecular mechanism underlying AGS is still unclear. Here, we show that RNase H2 is associated with a subset of genes, in a transcription-dependent manner where it interacts with RNA Polymerase II. RNase H2 depletion impairs transcription leading to accumulation of R-loops, structures that comprise RNA/DNA hybrids and a displaced DNA strand, mainly associated with short and intronless genes. Importantly, accumulated R-loops are processed by XPG and XPF endonucleases which leads to DNA damage and activation of the immune response, features associated with AGS. Consequently, we uncover a key role for RNase H2 in the transcription of human genes by maintaining R-loop homeostasis. Our results provide insight into the mechanistic contribution of R-loops to AGS pathogenesis.

ORGANISM(S): Homo sapiens

PROVIDER: GSE146970 | GEO | 2022/02/04

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2015-07-17 | GSE57353 | GEO
2023-12-25 | GSE240399 | GEO
2018-12-12 | E-MTAB-7087 | biostudies-arrayexpress
2014-11-03 | E-GEOD-53420 | biostudies-arrayexpress
2023-10-14 | GSE215896 | GEO
2023-04-18 | GSE221644 | GEO
| PRJNA612527 | ENA
2015-09-23 | GSE73334 | GEO
2022-03-26 | GSE199262 | GEO
2022-01-17 | GSE193710 | GEO