Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Expression data from D. melanogaster raised on glucose-enriched or unmodified holidic food

ABSTRACT: We used RNA-seq to determine transcriptional profiles of whole flies raised on either a glucose-enriched or unmodified holidic diet.

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE147222 | GEO | 2020/03/20

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Expression data from D. melanogaster raised on glucose-enriched or unmodified holidic food II

Project description:We used microarrays to examine transcriptional profiles of female flies raised on either a glucose-enriched (100 g/L) or unmodified holidic diet. We found several diet-dependent effects including increased growth pathway and immunity expression in flies raised on a glucose-enriched diet.

2020-03-20 | GSE147237 | GEO

Expression data from D. melanogaster raised on glucose-enriched or unmodified holidic food

Project description:Expression data from D. melanogaster raised on glucose-enriched or unmodified holidic food

| PRJNA613451 | ENA

Expression data from D. melanogaster raised on glucose-enriched or unmodified holidic food II

Project description:Expression data from D. melanogaster raised on glucose-enriched or unmodified holidic food II

| PRJNA613482 | ENA

CD4+ T cells depletion affects gene expression in hippocampus of mice raised in enriched environment

Project description:CD4+ T cells depletion affects gene expression in hippocampus of mice raised in enriched environment Enriched Environment Mice Hippocampi CD4+ T cells depleted or not (4 replicates)

2019-06-04 | GSE115507 | GEO

Expression data from drosophila melanogaster

Project description:Space travel presents unlimited opportunities for exploration and discovery, but requires a more complete understanding of the immunological consequences of long-term exposure to the conditions of spaceflight. To understand these consequences better and to contribute to design of effective countermeasures, we used the Drosophila model to compare innate immune responses to bacteria and fungi in flies that were either raised on earth or in outer space aboard the NASA Space Shuttle Discovery (STS-121). Microarrays were used to characterize changes in gene expression that occur in response to infection by bacteria and fungus in drosophila that were either hatched and raised in outer space (microgravity) or on earth (normal gravity). Whole Oregon R strain drosophila melanogaster fruit flies either raised on earth or in space that were (1) uninfected, (2) infected with bacteria (Escherichia coli), or (3) infected with fungus (Beauveria bassiana) were used for RNA extraction and hybridization on Affymetrix microarrays.

2013-12-11 | E-GEOD-53196 | biostudies-arrayexpress

Expression data from drosophila melanogaster

2013-12-11 | GSE53196 | GEO

Comparison of CD4+ and CD8+ T cells from spleen of mice raised in standard or in enriched environment.

Project description:C57BL6/J female mice were raised either in standard environment (SE) for 8 weeks after birth or were put in enriched environment (EE) at postnatal 4 weeks up to 8 weeks old. Mice were sacrificed at 8 weeks and spleen were harvested. CD4+ and CD8+ T cells were sorted by FACS and CD4+ or CD8+ T were profiled using a low-input protocol adapted from single-cell RNAseq.

2018-05-28 | GSE100584 | GEO

Decrypting the functional design of unmodified translation elongation factor P

Project description:Stalling of ribosomes due to consecutive proline motifs during polypeptide synthesis is a challenge faced by organisms across all kingdoms. To overcome this, bacteria employ translation elongation factor P (EF-P), while archaea and eukaryotes rely on a/eIF5A. Typically, these elongation factors become active only after undergoing post-translational modifications (PTMs) such as ß-lysinylation, (deoxy-)hypusinylation, rhamnosylation, or 5-aminopentanolyation. An exception to this rule is found in EF-P members of the PGKGP-subfamily, which remain unmodified. However, the mechanism behind the ability of certain bacteria to bypass metabolically and energetically costly PTMs, thus retaining active EF-P, remains unclear. In this study, we investigated the design principles governing the full functionality of unmodified EF-Ps in Escherichia coli. We first screened for naturally unmodified EF-Ps that are active in an E. coli reporter strain. We identified EF-P from Rhodomicrobium vannielii capable of rescuing the growth deficiencies and changes in the proteome of E. coli ΔepmA mutant lacking the gene for the modifying EF-P-(R)-ß-lysine ligase. We then identified specific amino acids in domain I of the unmodified EF-P variant that affected its activity. Ultimately, we transferred these functional properties to other marginally active members of the PGKGP EF-P subfamily, resulting in fully functional unmodified variants in E. coli. These results have not only implications for the improved heterologous expression of polyproline-containing proteins in E. coli but offer applications for other bacterial hosts. Understanding the mechanisms that underlie the functionality of unmodified EF-P provides insights into cellular adaptations to optimize protein synthesis.

2024-04-08 | PXD044929 | Pride

Proteomic analysis of germ-free Drosophila melanogaster

Project description:A method for the long-term maintenance of germ-free flies was established using aseptic isolators. The methodology effectively and reliably yields large numbers of germ-free flies in homogeneous cultures. Germ-free flies exhibited increased lifespan (only female flies) and decreased egg production, markedly reduced fat storage, less midday sleep, and enhanced aggressiveness (male flies). Fructilactobacillus—a species of fly intestinal microbes—was re-colonized in germ-free flies, and these gnotobiotic flies were successfully maintained for numerous generations. The proteome of those flies were analyzed.

2024-10-02 | PXD045847 | JPOST Repository

Fecal samples from pasture-raised pigs

Project description:Fecal samples from pasture-raised pigs Metagenome

| PRJNA857725 | ENA