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Expression data from D. melanogaster raised on glucose-enriched or unmodified holidic food

ABSTRACT: Expression data from D. melanogaster raised on glucose-enriched or unmodified holidic food

PROVIDER: PRJNA613451 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR11350614_1.fastq.gz	Fastqsanger.gz
	SRR11350614_2.fastq.gz	Fastqsanger.gz
	SRR11350615_1.fastq.gz	Fastqsanger.gz
	SRR11350615_2.fastq.gz	Fastqsanger.gz
	SRR11350616_1.fastq.gz	Fastqsanger.gz

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Expression data from D. melanogaster raised on glucose-enriched or unmodified holidic food

Project description:We used RNA-seq to determine transcriptional profiles of whole flies raised on either a glucose-enriched or unmodified holidic diet.

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Expression data from D. melanogaster raised on glucose-enriched or unmodified holidic food II

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Expression data from D. melanogaster raised on glucose-enriched or unmodified holidic food II

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Project description:Ambiguity regarding the role of glucose-dependent insulinotropic polypeptide (GIP) in obesity arises from conflicting reports asserting that both GIP receptor (GIPR) agonism and antagonism are effective strategies for inhibiting weight gain. To enable identification and manipulation of Gipr-expressing (Gipr) cells we created GIPR-Cre knock-in mice. As GIPR-agonists have recently been reported to suppress food intake we aimed to identify central mediators of this effect. Gipr cells were identified in the arcuate, dorsomedial, and paraventricular nuclei of the hypothalamus, as confirmed by RNAscope in mouse and human. Single cell RNAseq identified clusters of hypothalamic Gipr cells exhibiting transcriptomic signatures for mural, glial and neuronal cells, the latter expressing somatostatin, but little proopiomelanocortin or agouti-related peptide. Activation of Gq-DREADDs in hypothalamic Gipr cells suppressed food intake in vivo, which was not obviously additive with concomitant GLP1R activation. These data identify hypothalamic GIPR as a target for the regulation of energy balance.

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