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Yeast genomic expression patterns in response to deoxynivalenol

ABSTRACT: The assessment of toxicity about deoxynivalenol (DON) using yeast gene expression comparison analysis. Two yeast strains, parental BY4743 and PTC1 mutant, were used for this study. SDS was used to raise the penetration of the yeast cell membrane. Keywords: stress response

ORGANISM(S): Schizosaccharomyces pombe Saccharomyces cerevisiae

PROVIDER: GSE14748 | GEO | 2010/05/31

SECONDARY ACCESSION(S): PRJNA112235

REPOSITORIES: GEO

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Yeast genomic expression patterns in response to deoxynivalenol

Project description:The assessment of toxicity about deoxynivalenol (DON) using yeast gene expression comparison analysis. Two yeast strains, parental BY4743 and PTC1 mutant, were used for this study. SDS was used to raise the penetration of the yeast cell membrane. Keywords: stress response Four conditions are compared with three replicates each. Each of the two strains was grown in either 0.01% SDS-containing YPD media or 0.01% SDS-containing YPD media with 25 ppm of deoxynivalenol.

2010-06-08 | E-GEOD-14748 | biostudies-arrayexpress

PTC1 mutant Yeast Genomic Expression Patterns in Response to deoxynivalenol

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PTC1 mutant Yeast Genomic Expression Patterns in Response to deoxynivalenol

Project description:The evaluation of the toxicity of deoxynivalenol using yeast gene expressions. Yeast PTC1 mutant strain was used for this study. SDS was used to raise the penetration of the yeast cell membrane. Keywords: stress response Two conditions were compared by the gene expression profiles. Both samples were grown in 0.01% SDS contained YPD media, and one of them was also supplied with 50 ppm of deoxynivalenol.

2011-07-21 | E-GEOD-20378 | biostudies-arrayexpress

Yeast Genomic Expression Patterns of PTC1 null mutant in Response to Aflatoxin B1 (AFB1)

Project description:The evaluation of the toxicity of Aflatoxin B1 using yeast gene expressions. Yeast strain; BY4743 PTC1 mutant, was used for this study. SDS was used to raise the penetration of the yeast cell membrane.

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Yeast Genomic Expression Patterns of Wild-type and PTC1 null mutant in Response to Aflatoxin B1 (AFB1)

Project description:The evaluation of the toxicity of Aflatoxin B1 using yeast gene expressions. Two yeast strains; parental BY4743 and PTC1 mutant, were used for this study. SDS was used to raise the penetration of the yeast cell membrane. Two conditions are compared with three replicates each. Both strains were grown in 0.01% SDS containing YPD media or 0.01% SDS, 25 ppm ABF1 containing YPD media. Publication can be found at http://www.cbi.or.jp/cbi/CBIj/vol9/9_94-E.pdf.

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Ribosome quality control is a central protection mechanism for yeast exposed to Deoxynivalenol and Trichothecin

Project description:Trichothecenes, like deoxynivalenol (DON) are secondary metabolites of Fusarium species, and are major pollutants in cereal food and feed products. They inhibit eukaryotic protein synthesis, however with only partly resolved consequences for the cellular homeostasis. Here we report a systematic functional investigation of the effects of DON on exponential phase growing yeast cells using either short doses of DON or continuos treatment with DON.

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Yeast genomic expression patterns in response to type B trichothecenes

Project description:The evaluation of mycotoxicity of type B trichothecenes using a yeast gene expression comparison analysis. The yeast BY4743 derivative PDR5 mutant was used for this study. The yeast cells were treated with trichothecene mycotoxins, and incubated at 25 degree for two hours, respectively. Total RNA was isolated with commercial kit (FastRNA Pro Red kit, Q-Biogene), and amplified RNA (aRNA) was synthesized with 3'IVT Express kit (Affymetrix). All samples were hybridized with Gene Chip (Yeast Genome 2.0 Array, Affymetrix), then each array chip was scanned by GeneChip Sanner 3000 (Affymetrix). The yeast strain was grown in YPD medium with 25 ppm of trichothecene mycotoxin (deoxynivalenol (DON), 3-acetyldeoxynivalenol (3ADON), 15-acetyldeoxynivalenol (15ADON), nivalenol (NIV), or fusarenone-X (FusX)). The same volume of DMSO was added to the control samples. The conditioned samples were normalized with three replicates.

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Deoxynivalenol affects the gene expression of A549 lung epithelial cells

Project description:Deoxynivalenol (DON) is an important Fusarium toxin of concern for food safety. The inhalation of powder contaminated with deoxynivalenol is possible and may cause lung toxicity. In this study, we investigated the gene expression profile of A549 cells treated with 0.2 microg/mL deoxynivalenol by microarray analysis. In total, 16 genes and 5 noncoding RNAs were significantly affected by deoxynivalenol treatment. 4 samples; 2 samples from nontreated cells and other 2 samples from 0.2 microg/mL DON-treated cells for 24 hours

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Deoxynivalenol affects the gene expression of A549 lung epithelial cells

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Salmonella Typhimurium: control vs. Deoxynivalenol (DON) treatment

Project description:Transcriptional profiling of log and stationary phase S. Typhimurium, comparing untreated controls with Deoxynivalenol treated samples. Each array used labelled cDNA against a common genomic DNA reference. Triplicate arrays were carried out for each of the 4 conditions: untreated log phase, untreated stationary phase, DON treated log phase and DON treated stationary phase

2011-05-20 | E-GEOD-29399 | biostudies-arrayexpress

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