Inhibition of PRC2 activity by H3K36 methylation
Ontology highlight
ABSTRACT:
ORGANISM(S): Drosophila melanogaster
PROVIDER: GSE148254 | GEO | 2020/11/28
REPOSITORIES: GEO
Similar Datasets
Project description:This is a dataset generated by the Drosophila Regulatory Elements modENCODE Project led by Kevin P. White at the University of Chicago. It contains ChIP-chip data on Agilent 244K dual-color arrays for antibody: H3K27me3 at 12 different time-points of Drosophila development. Current Dataset: [GSM384769..GSM384777]: ChIP-chip of H3K27me3 in Drosophila embryos at 0-4 hours of development [GSM384778..GSM384786]: ChIP-chip of H3K27me3 in Drosophila embryos at 4-8 hours of development [GSM384787..GSM384795]: ChIP-chip of H3K27me3 in Drosophila embryos at 12-16 hours of development [GSM384797..GSM384805]: ChIP-chip of H3K27me3 in Drosophila embryos at 16-20 hours of development [GSM384807..GSM384815]: ChIP-chip of H3K27me3 in Drosophila embryos at 20-24 hours of development [GSM384817..GSM384825]: ChIP-chip of H3K27me3 in Drosophila L1 larvae [GSM384826..GSM384834]: ChIP-chip of H3K27me3 in Drosophila L2 larvae [GSM384839..GSM384847]: ChIP-chip of H3K27me3 in Drosophila pupae [GSM385341..GSM385349]: ChIP-chip of H3K27me3 in Drosophila L3 larvae [GSM418225..GSM418233]: ChIP-chip of H3K27me3 in Drosophila Adult male [GSM418239..GSM418247]: ChIP-chip of H3K27me3 in Drosophila embryos at 8-12 hours of development [GSM442006..GSM442014]: ChIP-chip of H3K27me3 in Drosophila Adult female For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf
2009-03-28 | GSE15423 | GEO
Project description:This is a dataset generated by the Drosophila Regulatory Elements modENCODE Project led by Kevin P. White at the University of Chicago. It contains ChIP-chip data on Agilent 244K dual-color arrays for antibody: H3K27me3 at 12 different time-points of Drosophila development. Current Dataset: [GSM384769..GSM384777]: ChIP-chip of H3K27me3 in Drosophila embryos at 0-4 hours of development [GSM384778..GSM384786]: ChIP-chip of H3K27me3 in Drosophila embryos at 4-8 hours of development [GSM384787..GSM384795]: ChIP-chip of H3K27me3 in Drosophila embryos at 12-16 hours of development [GSM384797..GSM384805]: ChIP-chip of H3K27me3 in Drosophila embryos at 16-20 hours of development [GSM384807..GSM384815]: ChIP-chip of H3K27me3 in Drosophila embryos at 20-24 hours of development [GSM384817..GSM384825]: ChIP-chip of H3K27me3 in Drosophila L1 larvae [GSM384826..GSM384834]: ChIP-chip of H3K27me3 in Drosophila L2 larvae [GSM384839..GSM384847]: ChIP-chip of H3K27me3 in Drosophila pupae [GSM385341..GSM385349]: ChIP-chip of H3K27me3 in Drosophila L3 larvae [GSM418225..GSM418233]: ChIP-chip of H3K27me3 in Drosophila Adult male [GSM418239..GSM418247]: ChIP-chip of H3K27me3 in Drosophila embryos at 8-12 hours of development [GSM442006..GSM442014]: ChIP-chip of H3K27me3 in Drosophila Adult female For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf For each combination of time-point and antibody, triplicate ChIP experiments have been performed and hybridized on Agilent 244K arrays. 3 arrays per genome have been used so that each time-point is a set of 9 tiling arrays.
2010-06-22 | E-GEOD-15423 | biostudies-arrayexpress
Project description:Inhibition of PRC2 activity by H3K36 methylation
| PRJNA623606 | ENA
Project description:Study of genomic distribution of H3K27me3 in Drosophila melanogaster Myb mutant wing discs of 3rd instar larvae compared to control animals.
2020-03-12 | GSE100140 | GEO
Project description:The core Polycomb Repressor Complex 2 (PRC2) is composed of Ezh1/2, Suz12, Eed and is responsible for mediating both H3K27me2 and H3K27me3. However, the mechanisms by which PRC2 demarcates these two repressive modifications in the genome are unknown. In a functional screen, we identified the H3K36 dimethyltransferase Nsd1 as a modulator of PRC2-mediated di- and trimethylation of H3K27. ChIP-Seq analysis following the depletion of Nsd1 revealed a global reduction in H3K36me2 and an increase in H3K27me3 at sites previously marked by H3K27me2. We show that the H3K36me2 at H3K27me2 marks co-occupy regions and provide evidence that the presence of H3K36me2 functions to restrict the spatial distribution of Polycomb mediated H3K27me3 domains.
2018-09-15 | GSE86925 | GEO
Project description:The core Polycomb Repressor Complex 2 (PRC2) is composed of Ezh1/2, Suz12, Eed and is responsible for mediating both H3K27me2 and H3K27me3. However, the mechanisms by which PRC2 demarcates these two repressive modifications in the genome are unknown. In a functional screen, we identified the H3K36 dimethyltransferase Nsd1 as a modulator of PRC2-mediated di- and trimethylation of H3K27. ChIP-Seq analysis following the depletion of Nsd1 revealed a global reduction in H3K36me2 and an increase in H3K27me3 at sites previously marked by H3K27me2. We show that the H3K36me2 at H3K27me2 marks co-occupy regions and provide evidence that the presence of H3K36me2 functions to restrict the spatial distribution of Polycomb mediated H3K27me3 domains.
2018-03-29 | GSE107773 | GEO
Project description:H3K27 methylation mediated by the histone methyltransferase complex PRC2 is critical for transcriptional regulation, Polycomb silencing, Drosophila segmentation, mammalian X chromosome inactivation, and cancer. PRC2-mediated H3K27 methylation can spread along the chromatin and propagate the repressive chromatin environment; thus, chromatin components that antagonize the activity of PRC2 are important for restraining Polycomb silencing. Here we report that in HeLa cells, H3 histones unmethylated at Lys-36 are mostly methylated at Lys-27, with the exception of newly synthesized H3. In addition, K27me3 rarely co-exists with K36me2 or K36me3 on the same histone H3 polypeptide. Moreover, PRC2 activity is greatly inhibited on nucleosomal substrates with preinstalled H3K36 methylation. These findings collectively identify H3K36 methylation as a chromatin component that restricts the PRC2-mediated spread of H3K27 methylation. Finally, we provide evidence that the controversial histone lysine methyltransferase Ash1, a known Trithorax group protein that antagonizes Polycomb silencing in vivo, is an H3K36-specific dimethylase, not an H3K4 methylase, further supporting the role of H3K36 methylation in antagonizing PRC2-mediated H3K27 methylation.
| S-EPMC3048685 | biostudies-literature
Project description:PRC2-isogenic human malignant peripheral nerve sheath tumor (MPNST) M3 cells were generated through CRISPR/Cas9-mediated knockout of the PRC2 core component, SUZ12. PRC2 loss leads to global loss of H3K27me3, which causes H3K27ac redistribution and also affect other histone modification, e.g., H3K36me2/3.
2022-08-17 | GSE202555 | GEO
Project description:We investigated genome wide distribution of H3K36me2, H3K36me3 and H3K27me3 in the presence and absence of MMSET protein. MMSET overexpression in t(4;14)+ myeloma leads to global loss redistribution of H3K36me2 and genome-wide loss of H3K27 methylation. Despite the gloal decrease in H3K27me3, specific regions of the genome show enhanced H3K27me3 enrichment through increased recruitment of EZH2 methyltransferase ChIP-seq for H3K36me2, H3K36me3 and H3K27me3 in two cell types
2014-09-01 | E-GEOD-57977 | biostudies-arrayexpress
Project description:This is a dataset generated by the Drosophila Regulatory Elements modENCODE Project led by Kevin P. White at the University of Chicago. It contains ChIP-chip data on Agilent 244K dual-color arrays for H3K4me3 at 12 different time-points of Drosophila development. Current Dataset: [GSM385397..GSM385405]: ChIP-chip of H3K4me3 in Drosophila embryos at 12-16 hours of development [GSM386032..GSM386040]: ChIP-chip of H3K4me3 in Drosophila embryos at 0-4 hours of development [GSM386088..GSM386096]: ChIP-chip of H3K4me3 in Drosophila embryos at 4-8 hours of development [GSM386097..GSM386105]: ChIP-chip of H3K4me3 in Drosophila embryos at 16-20 hours of development [GSM386106..GSM386114]: ChIP-chip of H3K4me3 in Drosophila embryos at 20-24 hours of development [GSM386115..GSM386123]: ChIP-chip of H3K4me3 in Drosophila L1 larvae [GSM386125..GSM386133]: ChIP-chip of H3K4me3 in Drosophila L2 larvae [GSM386996..GSM387004]: ChIP-chip of H3K4me3 in Adult Male [GSM418254..GSM418262]: ChIP-chip of H3K4me3 in Drosophila embryos at 8-12 hours of development [GSM442287..GSM442292]: ChIP-chip of H3K4me3 in Pupae [GSM442293..GSM442301]: ChIP-chip of H3K4me3 in Adult Female [GSM442302..GSM442310]: ChIP-chip of H3K4me3 in Drosophila L3 larvae For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf
2009-03-28 | GSE15424 | GEO