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RNA-seq of AKP organoids over different stages of differentiation

ABSTRACT: Transcriptomic profiling of mouse tumoroids at early (2 days of Apc restoration) versus late stage differentiation (7 days of Apc restoration)

ORGANISM(S): Mus musculus

PROVIDER: GSE148352 | GEO | 2020/05/22

REPOSITORIES: GEO

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Expression data from mouse intestinal organoids I

Project description:APC inactivation is the early process in the tumorigenesis of colorectal cancer. We established organoid cultures from intestines of genetically modifeid mice harboring Apcfl/fl and R26CreERT2 allele We used microarrays to determine the alterations in intestinal stem cells in response to APC inactivation. Organoids were treated with 4OHT to induce recombination of APC or left untreated, and collected at 4 or 6 days after treatment. RNA were extracted and hybridization on Affymetrix microarrays.

2016-03-31 | E-GEOD-76847 | biostudies-arrayexpress

Intestinal IKKa is required for tumor initiation in APC mutant mice

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Organoids model distinct Vitamin E effects at different stages of prostate cancer evolution.

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RNA-seq of AKP organoids over different stages of differentiation

Project description:RNA-seq of AKP organoids over different stages of differentiation

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APC and P53 mutations synergize to create a therapeutic vulnerability to NOTUM inhibition in advanced colorectal cancer [scRNA-seq]

Project description:We thought the immediate effects of APC inactivation may represent unexplored therapeutic vulnerabilities in later stages of advanced colorectal adenocarcinoma. We performed transcriptome profiling of epithelial colon organoids immediately after APC inactivation and cross-referenced gene expression changes to the transcriptomes of several mouse models of colon cancer, including colon tumors in an inflammation-induced CRC model (AOM-DSS), a model of familial adenomatous polyposis (FAP, the Apcmin mouse), and an implantation-based model of metastatic adenocarcinoma using Apc/Trp53/KrasG12D/Smad4 quadruple-mutant tumor organoids15 (APKS tumoroids). Of the roughly 150 genes whose expression is acutely induced upon APC loss and remains elevated in these colon cancer models.

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Gene expression changes in Apc-mutant mouse intestinal organoids with and without deleting the Prox1 transcription factor

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