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Endophytic and rhizophytic prokaryotic communities in S. alterniflora and petroleum degradation

ABSTRACT: The experiment was designed to test the interactions of Spartina alterniflora, its microbiome, and the interaction of the plant-microbe relationship with oil from the Deepwater Horizon oil spill (DWH). Total RNA was extracted from leaf and root microbiome of S. alterniflora in soils that were oiled in DWH oil spill with or without added oil, as well as those grown in unoiled soil with or without added oil. The work in its entirety characterizes the transport, fate and catabolic activities of bacterial communities in petroleum-polluted soils and within plant tissues.

ORGANISM(S): uncultured bacterium Archaea synthetic construct Eukaryota Bacteria Viruses

PROVIDER: GSE148431 | GEO | 2020/04/10

REPOSITORIES: GEO

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Transcriptome response to the Deepwater Horizon oil spill identifies novel candidate genes for oil tolerance in natural populations of the foundation plant Spartina alterniflora

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Deepwater Horizon oil spill sediments

Project description:Deepwater Horizon oil spill sediments Metagenome

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Project description:Deepwater Horizon Oil Spill (DHOS) plume metatranscriptomes

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2020-12-22 | PXD021108 | Pride

Regulation of microRNAs in response to Deepwater Horizon oil in mahi-mahi (Coryphaena hippurus) embryos at 3 different stages.

Project description:Purpose:To help identify the role miRNAs in molecular mechanisms and pathways potentially involved in the developmental toxicity for fish exposed to Deepwater Horizon (DWH) oil, miRNA-mRNA networks in mahi-mahi (Coryphaena hippurus) embryos exposed to different DWH oils (source and artificially weathered oil) were evaluated at different critical windows of development using High Throughput Sequencing (HTS). Methods:miRNA profiles of 24, 48, 96 hpf mahi-mah larvae after slick and source oil exposure were generated by deep sequencing, in triplicate, using Illumina NextSeq v2. qRT–PCR validation was performed using SYBR Green assays. Results: Exposure of embryos to slick oil resulted in more DE miRNAs than source oil at all developmental stages 24hpf, 48hpf, and 96hpf. There was also an increase in the number of DE miRNA as development progressed, with 96hpf having the highest number of DE miRNAs. The expression of miRNAs and their target mRNA was further compared using advanced bioinformatics with subsequent target organ predictions based on their interactions. Gene ontology (GO) analysis on the target mRNAs was consistent with pathway analysis of miRNAs, predicting disruption of cardiovascular system development after oil exposure and showed that specific miRNA–mRNA interactions may contribute to these effects.

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