Transcriptomics

Dataset Information

0

Synthetic circular miR-21 RNA decoys enhance tumor suppressor expression and impair tumor growth in mice (RNA-Seq)


ABSTRACT: Natural and synthetic circular RNAs effectively impair miRNA function. MiR-21-5p is a potent oncomiR presenting ~33% of all miRNAs across cancers, ~41% in lung adenocarcinoma (LUAD), and ~68% in LUAD-derived cells. We validate and identify five main tumor suppressors targeted by miR-21-5p by deletion of the MIR21 locus in LUAD cells. Synthetic, liposome-delivered circular miR-21-5p decoys enhance expression of these tumor suppressors and severely impair tumor cell vitality at low doses. Decoy efficacy is not increased by bulging of miR-21-5p targeting sites, but associated with substantial cellular decoy stability, indicated by a half-life of ~20h. The intraperitoneal application of nanoparticle-delivered miR-21-5p decoys significantly impairs tumor growth in mouse LUAD tumor models. Decoys are well tolerated and were enriched in lung tissue. However, despite low decoy abundance, tumor suppressor expression was only increased in subcutaneous tumors. These findings suggest nanoparticle-delivered circular miRNA decoys as potent therapeutics in cancer treatment.

ORGANISM(S): Homo sapiens

PROVIDER: GSE148684 | GEO | 2020/07/20

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2020-07-20 | GSE148735 | GEO
2020-07-20 | GSE148734 | GEO
2020-03-02 | PXD016803 | Pride
| PRJNA625524 | ENA
| PRJNA625337 | ENA
| PRJNA625529 | ENA
| PRJNA625521 | ENA
2022-04-08 | GSE192656 | GEO
2012-02-28 | E-GEOD-35749 | biostudies-arrayexpress
2023-12-01 | GSE190461 | GEO