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Genetic ablation of either G6P-isomerase or dual LDHA/B in colon cancer cells LS174T , +/- MPP+ (1-methyl-4-phenylpyridinium)

ABSTRACT: In this study we explore the genomic re-wiring of a human colon cancer cell line (LS174T) in which fermentative glycolysis has been fully suppressed by CRISPR/Cas9 to disrupt glucose-6-phosphate isomerase (GPI-KO) or dual disruption of lactate dehydrogenases A and B (LDHAB-DKO). The effects of both KOs, had no effect on loss of viable cell function, corresponding to high concentrations of ATP production vs WT. All three clones were evaluated for transcriptomic changes using GeneChip™ Human Gene 2.1 ST Arrays by Affymetrix Inc assessing for mRNAs and long intergenic non-coding RNA transcripts.

ORGANISM(S): Homo sapiens

PROVIDER: GSE149289 | GEO | 2020/04/25

REPOSITORIES: GEO

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Genetic ablation of either G6P-isomerase or dual LDHA/B in colon cancer cells LS174T , +/- MPP+ (1-methyl-4-phenylpyridinium)

Project description:Genetic ablation of either G6P-isomerase or dual LDHA/B in colon cancer cells LS174T , +/- MPP+ (1-methyl-4-phenylpyridinium)

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2018-12-31 | GSE118048 | GEO

Gene expression profile upon IL-22 stimulation, forced expression of Hes1, or IL-22 stimulation under forced expression of Hes1, in human colon carcinoma-derived LS174T cells

Project description:A human colon carcinoma-derived cell line LS174T was modified to overexpress Hes1, a bHLH-type transcription factor, upon doxycycline addition (designated as LS174T-tetON-Hes1 cells), using the T-rex system (Invitrogen). We have previously shown that these cells can overexpress Hes1 under the control of CMV promoter (Zheng et al, Inflamm Bowel Dis, 17;2251-2260, 2011), and the amont of the overexpressed Hes1 protein reaches to the maximal level in early as 3 hours from doxycycline addition (100ng/ml), which persists for up to 24 hours. In the present experiment, LS174T-tetON-Hes1 cells were treated with recombinant human IL-22 (20ng/ml) alone, doxycycline alone (100ng/ml), co-treated by both IL-22 and doxycycline, or left untreated (Control) for 24 hours, and subjected for analysis. Experiment was done using a modified sub-line of LS174T cell (LS174T-tetON-Hes1 cells), in which overexpression of Hes1 can be induced by a Doxycycline dependent manner.

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Gene expression profile of ATF6a and ATF6b overexpression in LS174T cells

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2012-01-07 | E-GEOD-34926 | biostudies-arrayexpress

Transcription profiling of human carcinoma LS174T cells following depletion of Myc and Usp28 using shRNA

Project description:Analysis of gene expression following depletion of Myc and Usp28 using shRNA in colon carcinoma LS174T cells.

2007-04-19 | E-MEXP-1070 | biostudies-arrayexpress

Expression profile of splenocytes with glucose-6-phosphate isomerase (GPI) induced arthritis

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