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Next generation sequencing of chicken otocyst with pharmacologically induced loss-of-Wnt signalling

ABSTRACT: Bulk RNA-sequencing of individual E2.5 chicken otocyst incubated in media enriched with Wnt signalling inhibitor IWR-1 or control condition (DMSO) for 24h. Transcripts abundance was mapped to a chicken reference genome using Kallisto package and differentially expressed genes were identify using Sleuth package (genes with p-value < 0.05 were considered as significant). The expression of 1340 genes was significantly changed:733 genes were up-regulated and 607 were down-regulated. Biological Function enrichment analysis (Toppgene online tool) showed that many of these genes were associated with neurogenesis.

ORGANISM(S): Gallus gallus

PROVIDER: GSE149310 | GEO | 2020/12/01

REPOSITORIES: GEO

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Next generation sequencing of chicken otocyst with pharmacologicaly induced loss-of-Wnt signalling

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