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Next generation sequencing of chicken otocyst with genetically induced loss-of-Wnt signalling

ABSTRACT: Bulk RNA-sequencing of individual E3 chicken otocyst, in which Wnt signaling activity was reduced by genetic manipulation. The E2 otic placodes were in ovo transfected with a construct overexpressing truncated form of B-catenin reducing Wnt activity or a control plasmid expressing mCherry and collected 24h later. Transcripts abundance was mapped to a chicken reference genome using Kallisto package and differentially expressed genes were identify using Sleuth package (genes with p-value < 0.05 were considered as significant).

ORGANISM(S): Gallus gallus

PROVIDER: GSE230083 | GEO | 2023/11/13

REPOSITORIES: GEO

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Next generation sequencing of chicken otocyst with pharmacologicaly induced loss-of-Wnt signalling

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