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Importance of RAD6B in activation of canonical WNT signaling in melanoma development

ABSTRACT: We have demonstrated that RAD6B, a ubiquitin conjugating enzyme that is a principal component of translesion synthesis pathway, stabilizes and transcriptionally activates beta-catenin by lysine 63-linked polyubiquitination. RAD6B is also a beta-catenin transcriptional target, triggering a positive feedback loop. Canonical WNT signaling is critical for melanocyte lineage commitment and melanoma development, and RAD6B and beta-catenin are co-expressed in primary and metastatic melanomas. To understand how RAD6B influences melanoma development/progression, we performed RNA-seq of human metastatic M14 melanoma cells, their RAD6B knockout counterpart, and human HEMa-LP normal melanocytes.

ORGANISM(S): Homo sapiens

PROVIDER: GSE149390 | GEO | 2020/10/27

REPOSITORIES: GEO

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