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Gene expression in WT and LRF-deficient thymic IEL precusors (IELp) and their progeny CD8aa IEL

ABSTRACT: Gene expression of IELp and CD8aa IEL was determined by RNAseq

ORGANISM(S): Mus musculus

PROVIDER: GSE149943 | GEO | 2022/03/30

REPOSITORIES: GEO

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Single-cell Gene expression of WT CD8aa IEL and LRF-deficient CD8aa splenocytes

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Project description:Mouse small intestine intraepithelial lymphocytes (IEL) that express a ab TCR and CD8aa homodimers are an enigmatic T cell subset, as their specificity and in vivo function remain to be defined. To gain insight into the nature of these cells, we performed global gene expression profiling using microarray analysis, combined with PCR and flow cytometry to determine the level of expression of selected genes. Using these methods, TCR ab+ CD8aa IEL were compared to their TCR ab+ CD8b+ and TCR gd+ counterparts. Experiment Overall Design: In this study, the three IEL populations were isolated by cell sorting from a pool of IELs from 20 mice. RNA from each IEL subset was used to probe one microarray. A replicate experiment was performed on a seperate occasion using a new pool of 20 mice and three additional microarrays were used.

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