Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Single-cell Gene expression of WT and LRF-deficient thymic IEL precusors (IELp)

ABSTRACT: Single-cell Gene expression of WT and LRF-deficient thymic IEL precusors (IELp)

PROVIDER: PRJNA630733 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other
	SRR11713457.fastq.gz	Fastqsanger.gz
	SRR11713458.fastq.gz	Fastqsanger.gz
	SRR11713459.fastq.gz	Fastqsanger.gz
	SRR11713460.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 9

Similar Datasets

Gene expression in WT and LRF-deficient thymic IEL precusors (IELp) and their progeny CD8aa IEL

Project description:Gene expression in WT and LRF-deficient thymic IEL precusors (IELp) and their progeny CD8aa IEL

| PRJNA630671 | ENA

Single-cell Gene expression of WT CD8aa IEL and LRF-deficient CD8aa splenocytes

Project description:To examine how the cluster composition of CD8aa IEL and their transcriptomic signatures were affected by LRF disruption, single-cell gene expression of CD8aa IEL from control (Cd4 Cre–Lrffl/fl) and CD8aa splenocytes from LRF KO (Cd4 Cre+Lrffl/fl ) mice were determined by scRNAseq.

2022-03-30 | GSE186164 | GEO

Single-cell Gene expression of WT CD8aa IEL and LRF-deficient CD8aa splenocytes

Project description:Single-cell Gene expression of WT CD8aa IEL and LRF-deficient CD8aa splenocytes

| PRJNA772760 | ENA

Single-cell Gene expression of WT and LRF-deficient thymic IEL precusors (IELp)

Project description:To examine how the cluster composition of IELp and their transcriptomic signatures were affected by LRF disruption, single-cell gene expression of IELp from control (Cd4 Cre–Lrffl/fl) and LRF KO(Cd4 Cre+Lrffl/fl )mice were determined by scRNAseq

2022-03-30 | GSE149985 | GEO

Gene expression in WT and LRF-deficient thymic IEL precusors (IELp) and their progeny CD8aa IEL

Project description:Gene expression of IELp and CD8aa IEL was determined by RNAseq

2022-03-30 | GSE149943 | GEO

Single-cell ATAC of WT thymic IEL precursors (IELp)

Project description:Single-cell ATAC of WT thymic IEL precursors (IELp)

| PRJNA773138 | ENA

RNASeq comparing Thpok and LRF-deficient Tregs to Control

Project description:The transcription factor Thpok is required for intrathymic CD4+ T cell differentiation and, together with its homolog LRF, supports CD4+ T cell helper effector responses, However, it is not known if these factors are needed for the T regulatory arm of MHC-II responses. We inactivated the genes encoding both factors in differentiated Treg cells, to see whether how they are redundantly required to maintain the size and function of the post-thymic Treg pool and for immune homeostasis.

2017-08-07 | GSE85532 | GEO

Expression data from control and LRF-deficient mouse germinal center B cells

Project description:B cells are indispensable for humoral immunity, as they ultimately give rise to antibody-secreting plasma cells. During T cell-dependent antibody responses, naive B cells form germinal centers (GCs), a distinct histologic structure found in secondary lymphoid organs. Naive B cells become activated upon interaction with T cells and antigen presenting cells, and begin to rapidly proliferate and form the characteristic GC structure. To elucidate the overall effect of LRF loss in the GCB cell transcriptome, gene expression microarray analysis of FACS-sorted GCB cells was performed. LRF Flox/+ mb-1 Cre+ mice were used as a control to normalize the potential effects of Cre recombinase, and four RNA samples for each genotype were used for the analysis. B cell-specific LRF knockout (LRF Flox/Flox mb-1 Cre+) and control (LRF Flox/+ mb-1 Cre+) mice (4 each) were immunized with sheep red blood cells, and GCB cells were FACS-sorted 7 days later.

2011-04-08 | E-GEOD-28449 | biostudies-arrayexpress

Expression data from control and LRF (leukemia/lymphoma related factor)-deficient mouse LT-HSCs

Project description:LRF, which is encoded by the ZBTB7A gene and formerly known as POKEMON (POK erythroid myeloid ontogenic factor), was originally identified as a PLZF (promyelocytic leukemia zinc finger) homologue interacting with BCL6 (B-cell lymphoma 6). LRF is a transcription factor that is broadly expressed in hematopoietic lineage cells, but its expression is particularly high in erythroblasts and germinal center (GC) B-cells. The goal of this study is to assess the effect of LRF loss on the LT-HSC transcriptome. Nine days after injection of adult mice with polyinosinic polycytidylic acid (pIpc) to activate Cre, total RNAs were isolated from double-sorted LT-HSCs from LRF Flox/+ Mx1-Cre+ and LRF Flox/Flox Mx1-Cre+ mice and processed for microarray analysis. We performed gene expression microarray analysis of FACS-sorted LT-HSCs (LSK IL7Ra-Flt3-CD150+CD48-) to assess the effect of Lrf loss on the LT-HSC transcriptome. Zbtb7a Flox/+ Mx1-Cre+ mice were used as a control to normalize the potential effects of Cre recombinase. LT-HSCs were FACS-sorted from three Lrf knockout (Zbtb7a Flox/Flox Mx1-Cre+) and two control (Zbtb7a Flox/+ Mx1-Cre+) mice, nine days after the first pIpC injection.

2012-10-26 | E-GEOD-41839 | biostudies-arrayexpress

Expression data from control and LRF (leukemia/lymphoma related factor)-deficient mouse LT-HSCs

2012-10-26 | GSE41839 | GEO